Correlation between the HIF-1α/Notch signaling pathway and Modic changes in nucleus pulposus cells isolated from patients with low back pain

Correlation between the HIF-1α/Notch signaling pathway and Modic changes in nucleus pulposus cells isolated from patients with low back pain

Background: Line of HIF-1α / Notch signaling regulate cell proliferation, apoptosis, and metabolism of the intervertebral disc (IVD) and is involved in disc degeneration. The nucleus pulposus (NP) is an important structure adjacent to the IVD. However, the role of HIF-1α / Notch signaling pathway in NP cells obtained from patients with different Modic changes (MC) is still unclear. The purpose of this study was to investigate the role of HIF-1α and components of the Notch pathway in NP obtained from patients with various MC.


Methods: A total of 85 NP tissue samples collected from patients who underwent diskectomy for the treatment of low back pain. NP network were divided into four groups based on the adjacent endplate degeneration, namely, MC I, II, III, and negative MC group. Expression of HIF-1α and Notch-related component is measured and compared.


Results: The expression of HIF-1α, Notch1 and Notch2 gradually increased in the first MC and MC II group than in the negative group MC. HIF-1α and Notch-related components are rarely detected in MC group III.
Conclusion: The expression of HIF-1α / Notch increased in cells of patients with NP MC MC I and II. HIF-1α and components related to the potential biomarkers Notch and HIF-1α / Notch signaling pathway may serve as a promising therapeutic target for disc degeneration in patients with MC.

Correlation between the HIF-1α/Notch signaling pathway and Modic changes in nucleus pulposus cells isolated from patients with low back pain
Correlation between the HIF-1α/Notch signaling pathway and Modic changes in nucleus pulposus cells isolated from patients with low back pain

Targeting the Notch signaling pathway and TGF-β to prevent retinal fibrosis in vitro and in vivo

Rationale: Notch and transforming growth factor-β (TGFβ) two signaling pathways that control intracellular mechanisms of fibrosis in general, but if they play a major role in retinal fibrosis is less clear. Here we learn how these two signal pathways regulate cell Müller-dominated retinal fibrosis in vitro and in vivo. Methods: Human cells MIO-M1 Müller and TGFβ1 treated with Notch ligands, either alone or in combination. Western blot was conducted to study the changes protease γ-Secretase, Notch downstream effectors, endogenous TGFβ1, Smad3 phosphorylated (p-Smad3) and the extracellular matrix (ECM) proteins.

We also studied the effects RO4929097, a selective inhibitor of γ-Secretase, the ECM protein expression after ligand stimulation. Müller cell viability and cytotoxicity was studied by AlamarBlue to test the cytotoxicity of lactate. Finally, we studied the changes in Notch and TGFβ signaling and test the effect of an intravitreal injection of Notch pathway inhibitors RO4929097 on retinal fibrosis resulting from sodium iodate (NaIO3) -induced retinal injury in rats.

We also studied the security of RO4929097 intravitreal injection of normal mice. Results: Treatment of Muller cells with protease deregulated Notch ligand and Notch γ-Secretase downstream effector, with an increased expression of endogenous TGFβ1, TGFβ receptor and p-Smad3. TGFβ1 regulated expression of proteins associated with both signal paths in the same way. Notch ligands and TGFβ1 have an additive effect on ECM protein overexpression in Müller cells were inhibited by RO4929097. Notch and TGFβ ligand stimulates proliferation of Müller cells were inhibited by RO4929097 without damaging the cells. NaIO3-induced injury to the retina enabled both Notch and TGFβ signaling pathway in vivo intravitreal injection RO4929097 prevented gliosis Müller cells and inhibit excessive ECM proteins in this murine model.

Human Notch Signaling Pathway
HNOT-I 1 set
EUR 548
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA900A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA930A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools
Mouse Notch Signaling Primer Library
MNOT-I 1 set
EUR 548
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500
HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500
HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen
Array Designer
AD6 1
EUR 4248
Nylon Array
MVSNGA110 10 Slides
EUR 226
Nylon Array
MVSNGA120 20 Slides
EUR 363
Green qPCR SuperMix
20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.
Probe qPCR SuperMix
20-abx098040
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.
Universal qPCR Mixture
QPCR1000-UNIV 4X1.25ml
EUR 276.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens)
T8234708-2 2 slides
EUR 307
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens)
T8234708-5 5 slides
EUR 579
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Notch 1 antibody
20R-1749 100 ug
EUR 673
Description: Rabbit polyclonal Notch 1 antibody
Notch 1 antibody
70R-12158 100 ug
EUR 403
Description: Rabbit polyclonal Notch 1 antibody
Notch 1 antibody
70R-31090 100 ug
EUR 327
Description: Rabbit polyclonal Notch 1 antibody
Notch 2 antibody
70R-31091 100 ug
EUR 327
Description: Rabbit polyclonal Notch 2 antibody
Notch 1 Antibody
3881-100
EUR 316
Notch 1 Antibody
3881-30T
EUR 146
Notch 1 antibody
10R-6539 50 ug
EUR 192
Description: Mouse monoclonal Notch 1 antibody
Notch 1 Antibody
48766-100ul 100ul
EUR 333
Notch 1 Antibody
48766-50ul 50ul
EUR 239
Notch 1 Antibody
abx139789-01mg 0.1 mg
EUR 356
  • Shipped within 5-12 working days.
pGreenFire1-Notch (plasmid)
TR020PA-1 10 ug
EUR 786
  • Category: Lentiviral Technology
pGreenFire1-Notch (virus)
TR020VA-1 >2 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology
anti-Notch 2
YF-PA24259 50 ul
EUR 334
Description: Mouse polyclonal to Notch 2
Green qPCR SuperMix UDG
20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.
Top Green qPCR SuperMix
20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.
Tip Green qPCR SuperMix
20-abx098034
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.
Library Quantification qPCR SuperMix
20-abx098896
  • EUR 230.00
  • EUR 411.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.
qPCR Control Template Kit
G929 100 rxns
EUR 148
Eva QPCR SuperMix Kit
K5052200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Eva QPCR SuperMix Kit
K5052400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Pro QPCR SuperMix Kit
K5053200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Pro QPCR SuperMix Kit
K5053400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
QPCR Positive Control Kit
M1126-100
EUR 403
Neural Lineage qPCR Profiler
RA500A-1 10 reactions
EUR 999
  • Category: Lineage Profilers
Regulatory RNA qPCR Profiler
RA950A-1 20 rxn
EUR 880
  • Category: Long Non-coding RNA Tools
Lentivirus qPCR Quantification Kit
K1471-100 100 Rxns
EUR 298
Retrovirus qPCR Quantification Kit
K1472-100 100 Rxns Ask for price
Amino Particle Array Kit
APAK-3567-7K 7X1 mL
EUR 558
Description: Amino Particle Array KitGentaur offers a wide variety of uniform particles, ranging in size from 0.05 um to 20 um, with surface functional groups such as carboxyl or amino groups for covalent coupling of proteins or other ligands.
Fluorescent Particle Array Kit
FPAK-4058--10K 10 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Blue Particle Array Kit
PAK-3567-7K 7X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Blue Particle Array Kit
PAK-4067-8K 8X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Blue Particle Array Kit
PAK-5067-10K 10X1 mL
EUR 939
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Blue Particle Array Kit
PAK-5067-5A 5X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Blue Particle Array Kit
PAK-5067-5B 5X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Blue Particle Array Kit
PAK-7067-9K 9X1 mL
EUR 939
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
FDA Standard Tissue Array
T8234701-1 1 slides
EUR 214
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
FDA Standard Tissue Array
T8234701-5 5 slides
EUR 654
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
CA125 antibody array kit
VArk-Lsx008 1 mg
EUR 664
Description: CA125 can be used as a tumor marker to identify the type of tumor and check if the treatment is working.
Ferritin antibody array kit
VArk-Lsx009 1 mg
EUR 419
Description: Used for detection of Ferritin
Microalbuminuria antibody array kit
VArk-Lsx010 1 mg
EUR 419
Description: Used to detect early signs of kidney damage
Notch-1 (human) ELISA Kit
K4763-100 100 assays
EUR 809
  • Kit components:
  • Notch-1 Microplate (Item A) coated with anti-human Notch-1, 96 wells
  • Wash Buffer Concentrate (20x) (Item B)
  • Standards (Item C) (recombinant human Notch-1)
  • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.
  • Show more
Description: Sensitive, Colorimetric Assay
Notch-1 ELISA Kit| Mouse Neurogenic locus notch homolog protein
EF014010 96 Tests
EUR 689
Human Notch Homolog 1 ELISA kit
E01N0594-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Notch Homolog 1 ELISA kit
E01N0594-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Notch Homolog 1 ELISA kit
E01N0594-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Notch Homolog 4 (NOTCH4) Protein
20-abx166655
  • EUR 662.00
  • EUR 272.00
  • EUR 2040.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Human Notch Homolog 2 (NOTCH2) Protein
20-abx166840
  • EUR 676.00
  • EUR 286.00
  • EUR 2082.00
  • EUR 801.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Human Notch Homolog 3 (NOTCH3) Protein
20-abx168112
  • EUR 662.00
  • EUR 272.00
  • EUR 2026.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
TranslationBlocker Human Notch-1 siRNA, 10nmol
QX37-10nmol 10nmol
EUR 377
TranslationBlocker Human Notch-1 siRNA, 2nmol
QX37-2nmol 2nmol
EUR 276
cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.
HotTaq Probe qPCR Mix (ROX)
BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq Probe qPCR Mix (Capillary)
BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq Probe qPCR Universal Mix
BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq EvaGreen qPCR Mix (ROX)
BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq EvaGreen qPCR Mix (Capillary)
BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq EvaGreen qPCR Universal Mix
BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
SYBR Green qPCR Master Mix
HY-K0501 5 mL (500 rxns)
EUR 263
RT Master Mix for qPCR
HY-K0510 1 mL (100 rxns)
EUR 291
Power Eva QPCR SuperMix Kit
K5057200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Power Eva QPCR SuperMix Kit
K5057400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Methylamp MS-qPCR Fast Kit
P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible
BrightGreen 5X qPCR MasterMix-iCycler
MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213
TaqProbe 5X qPCR MasterMix-ROX
MasterMix-5P 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213
TaqProbe 5X qPCR MasterMix-iCycler
MasterMix-5PC 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213
TaqProbe 5X qPCR MasterMix-Multiplex
MasterMix-5PM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213
BrightGreen 5X qPCR MasterMix-ROX
MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213
BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140
BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376
KiloGreen 2X qPCR MasterMix-iCycler
MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140
KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140
BrightGreen miRNA qPCR MasterMix-iCycler
MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151
BrightGreen miRNA qPCR MasterMix-ROX
MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151
TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140
TaqProbe 2X qPCR MasterMix-iCycler
MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140
TaqProbe 2X qPCR MasterMix-Multiplex
MasterMix-PM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140
BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140
BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376
AceQ qPCR Probe Master Mix
Q112-02 500 rxn (20 μl/rxn)
EUR 221
AceQ qPCR Probe Master Mix
Q112-03 2500 rxn (20 μl/rxn)
EUR 646
Notch 1 Blocking Peptide
33R-10562 50 ug
EUR 349
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Notch 1 antibody, catalog no. 20R-1749
Notch 1 Blocking Peptide
33R-10970 50 ug
EUR 191
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Notch 1 antibody, catalog no. 70R-12158
Notch 1 Blocking Peptide
3881BP-50
EUR 153
Notch 3 antibody (PE)
61R-1343 100 ug
EUR 440
Description: Armenian Hamster monoclonal Notch3 antibody (PE)

We did not find any security issues until 17 days after intravitreal injection RO4929097. Conclusion: Inhibiting Notch signaling may be an effective way to prevent retinal fibrosis. This study is of clinical significance in developing treatments to prevent fibrosis in proliferative vitreoretinopathy, proliferative diabetic retinopathy and wet age-related macular degeneration.

Anthony

Related Posts

fallback-image

IMMUNOGLOBULINS

Increased Expression of Fibulin-1 Is Associated With Hepatocellular Carcinoma Progression by Regulating the Notch Signaling Pathway

Increased Expression of Fibulin-1 Is Associated With Hepatocellular Carcinoma Progression by Regulating the Notch Signaling Pathway

Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain

Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain

No Comment

Leave a Reply

Your email address will not be published. Required fields are marked *

Tags

December 2021
M T W T F S S
 12345
6789101112
13141516171819
20212223242526
2728293031  

Recent Posts

Categories

Pages