Background: Line of HIF-1α / Notch signaling regulate cell proliferation, apoptosis, and metabolism of the intervertebral disc (IVD) and is involved in disc degeneration. The nucleus pulposus (NP) is an important structure adjacent to the IVD. However, the role of HIF-1α / Notch signaling pathway in NP cells obtained from patients with different Modic changes (MC) is still unclear. The purpose of this study was to investigate the role of HIF-1α and components of the Notch pathway in NP obtained from patients with various MC.
Methods: A total of 85 NP tissue samples collected from patients who underwent diskectomy for the treatment of low back pain. NP network were divided into four groups based on the adjacent endplate degeneration, namely, MC I, II, III, and negative MC group. Expression of HIF-1α and Notch-related component is measured and compared.
Results: The expression of HIF-1α, Notch1 and Notch2 gradually increased in the first MC and MC II group than in the negative group MC. HIF-1α and Notch-related components are rarely detected in MC group III. Conclusion: The expression of HIF-1α / Notch increased in cells of patients with NP MC MC I and II. HIF-1α and components related to the potential biomarkers Notch and HIF-1α / Notch signaling pathway may serve as a promising therapeutic target for disc degeneration in patients with MC.
Correlation between the HIF-1α/Notch signaling pathway and Modic changes in nucleus pulposus cells isolated from patients with low back pain
Targeting the Notch signaling pathway and TGF-β to prevent retinal fibrosis in vitro and in vivo
Rationale: Notch and transforming growth factor-β (TGFβ) two signaling pathways that control intracellular mechanisms of fibrosis in general, but if they play a major role in retinal fibrosis is less clear. Here we learn how these two signal pathways regulate cell Müller-dominated retinal fibrosis in vitro and in vivo. Methods: Human cells MIO-M1 Müller and TGFβ1 treated with Notch ligands, either alone or in combination. Western blot was conducted to study the changes protease γ-Secretase, Notch downstream effectors, endogenous TGFβ1, Smad3 phosphorylated (p-Smad3) and the extracellular matrix (ECM) proteins.
We also studied the effects RO4929097, a selective inhibitor of γ-Secretase, the ECM protein expression after ligand stimulation. Müller cell viability and cytotoxicity was studied by AlamarBlue to test the cytotoxicity of lactate. Finally, we studied the changes in Notch and TGFβ signaling and test the effect of an intravitreal injection of Notch pathway inhibitors RO4929097 on retinal fibrosis resulting from sodium iodate (NaIO3) -induced retinal injury in rats.
We also studied the security of RO4929097 intravitreal injection of normal mice. Results: Treatment of Muller cells with protease deregulated Notch ligand and Notch γ-Secretase downstream effector, with an increased expression of endogenous TGFβ1, TGFβ receptor and p-Smad3. TGFβ1 regulated expression of proteins associated with both signal paths in the same way. Notch ligands and TGFβ1 have an additive effect on ECM protein overexpression in Müller cells were inhibited by RO4929097. Notch and TGFβ ligand stimulates proliferation of Müller cells were inhibited by RO4929097 without damaging the cells. NaIO3-induced injury to the retina enabled both Notch and TGFβ signaling pathway in vivo intravitreal injection RO4929097 prevented gliosis Müller cells and inhibit excessive ECM proteins in this murine model.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens)
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Amino Particle Array KitGentaur offers a wide variety of uniform particles, ranging in size from 0.05 um to 20 um, with surface functional groups such as carboxyl or amino groups for covalent coupling of proteins or other ligands.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: A sandwich ELISA for quantitative measurement of Human Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Armenian Hamster monoclonal Notch3 antibody (PE)
We did not find any security issues until 17 days after intravitreal injection RO4929097. Conclusion: Inhibiting Notch signaling may be an effective way to prevent retinal fibrosis. This study is of clinical significance in developing treatments to prevent fibrosis in proliferative vitreoretinopathy, proliferative diabetic retinopathy and wet age-related macular degeneration.
Human Notch Signaling Pathway  20 profiles)
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles  20 profiles)
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles  20 profiles)
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles 
Mouse Notch Signaling Primer Library 
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 
Array Designer 
Nylon Array 
Green qPCR SuperMix 
Probe qPCR SuperMix 
Universal qPCR Mixture )
Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens) 
Notch 1 antibody 
Notch 2 antibody 
Notch 1 Antibody )
pGreenFire1-Notch (plasmid) )
pGreenFire1-Notch (virus) 
anti-Notch 2 
Green qPCR SuperMix UDG 
Top Green qPCR SuperMix 
Tip Green qPCR SuperMix 
Library Quantification qPCR SuperMix 
qPCR Control Template Kit 
Eva QPCR SuperMix Kit 
Pro QPCR SuperMix Kit 
QPCR Positive Control Kit 
Neural Lineage qPCR Profiler 
Regulatory RNA qPCR Profiler 
Lentivirus qPCR Quantification Kit 
Retrovirus qPCR Quantification Kit 
Amino Particle Array Kit 
Fluorescent Particle Array Kit 
Blue Particle Array Kit 
FDA Standard Tissue Array 
CA125 antibody array kit 
Ferritin antibody array kit 
Microalbuminuria antibody array kit  ELISA Kit)
Notch-1 (human) ELISA Kit 
Notch-1 ELISA Kit| Mouse Neurogenic locus notch homolog protein 
Human Notch Homolog 1 ELISA kit  Protein)
Human Notch Homolog 4 (NOTCH4) Protein  Protein)
Human Notch Homolog 2 (NOTCH2) Protein  Protein)
Human Notch Homolog 3 (NOTCH3) Protein 
TranslationBlocker Human Notch-1 siRNA, 10nmol 
TranslationBlocker Human Notch-1 siRNA, 2nmol 
cDNA Synthesis SuperMix for qPCR )
HotTaq Probe qPCR Mix (ROX) )
HotTaq Probe qPCR Mix (Capillary) 
HotTaq Probe qPCR Universal Mix )
HotTaq EvaGreen qPCR Mix (ROX) )
HotTaq EvaGreen qPCR Mix (Capillary) 
HotTaq EvaGreen qPCR Universal Mix 
SYBR Green qPCR Master Mix 
RT Master Mix for qPCR 
Power Eva QPCR SuperMix Kit 
Methylamp MS-qPCR Fast Kit 
BrightGreen 5X qPCR MasterMix-iCycler 
TaqProbe 5X qPCR MasterMix-ROX 
TaqProbe 5X qPCR MasterMix-iCycler 
TaqProbe 5X qPCR MasterMix-Multiplex 
BrightGreen 5X qPCR MasterMix-ROX 
BrightGreen 2X qPCR MasterMix-iCycler 
KiloGreen 2X qPCR MasterMix-iCycler 
KiloGreen 2X qPCR MasterMix-ROX 
BrightGreen miRNA qPCR MasterMix-iCycler 
BrightGreen miRNA qPCR MasterMix-ROX 
TaqProbe 2X qPCR MasterMix-ROX 
TaqProbe 2X qPCR MasterMix-iCycler 
TaqProbe 2X qPCR MasterMix-Multiplex 
BrightGreen 2X qPCR MasterMix-ROX 
AceQ qPCR Probe Master Mix 
Notch 1 Blocking Peptide )
Notch 3 antibody (PE) 
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Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Equipments | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | quipments | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Uncategorized | Vector & Virus | Western Blot
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