Plastic changes in the anterior cingulate cortex (ACC) is very important in hypersensitivity to pain caused by peripheral nerve injury. The Notch signaling pathway has been shown to regulate differentiation and synaptic transmission. Therefore, this study was to investigate the function of Notch signaling pathway in the ACC during nociceptive transmission caused by neuropathy pain
.We adopt Western boltting, N- [N- (3,5-difluorophenacetyl) l-Alanyl] -S- Phenylglycine t-butyl ester (dAPT) microinjections, RNA interference targeting Notch1, hairy and enhancer of split (Hes) 1 or Hes5, electrophysiological recording and behavioral tests to verify the relationship between Notch signaling in the ACC and neuropathic pain in rats adult male Sprague Dawley .Levels of Notch intracellular domain (NICD) increased in the ACC at day 7 after chronic constriction injury (CCI) operation or spared nerve injury (SNI).
Meanwhile, the mRNA level of Notch signaling downstream effector Hes1 Hes5 mRNA increased while the rate unchanged. Injection of DAPT, a γ-Secretase (the key enzyme involved in the Notch pathway) inhibitors, ACC to significantly reversed neuropathic pain behavior. Intra-ACC injection of short hairpin RNA (shRNA) -Notch reduce the expression of NICD and lower potentiation of synaptic transmission in the ACC. In addition, the perception of pain is also addressed in mice undergoing CCI or SNI. This process is mainly mediated by Hes1 downstream effectors, but not Hes5.Based this result, activation of the Notch / Hes1 signaling pathway in the ACC participated in the development of neuropathic pain, suggesting that Notch pathway may be a new therapeutic target to treat chronic pain.
Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain
[Decrease in long chains MALAT1 non-coding RNA expression and increased expression of HSA-miR155-3p involved in the regulation of Notch signaling pathway in patients with rheumatoid arthritis]
Objective To investigate the expression of a long chain of non-coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) and HSA-miR155-3p in patients with rheumatoid arthritis (RA) and their relationship with the Notch signaling pathway, and explore the possibility of RA pathogenesis. Method peripheral blood of patients with RA (RA group) and healthy controls (group NC) was used to screen differentially expressed mRNA by lncRNA and high-throughput gene sequencing.
Reverse-transcription PCR is used to detect the expression of lncRNA MALAT1, HSA-miR155-3p and Notch signaling pathway receptor ligands. Results lncRNA sequencing analysis showed a total of 9158 lncRNAs are expressed differently. Gene ontology (GO) annotation of functional classification revealed that differential mRNA is expressed mainly involved in inflammatory immune response, cellular transcriptional regulation and so on.
path analysis proves that the differentially expressed mRNA was significantly associated with genes involved in rheumatoid arthritis, cellular aging, and the Notch signaling pathway. According to the prediction of cis and trans, Jagged1-2, Delta1-2 and Notch1-2 probably closely related to RA. MALAT1 the RA group was lower than in group NC, and HSA-miR155-3p expression was significantly higher than in the NC group.
Description: A competitive ELISA for quantitative measurement of Porcine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
Description: A competitive ELISA for quantitative measurement of Porcine Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Suppressor of cytokine signaling 2 (SOCS2) ELISA Kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 3 ELISA kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 3 ELISA kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G Protein Signaling 4 (RGS4) ELISA Kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator Of G Protein Signaling 4 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator Of G Protein Signaling 4 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: A competitive ELISA for quantitative measurement of Guinea pig WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The endoplasmic reticulum (ER) is an organelle composed of an interconnected network of tubules, vesicles and cisternae found in all eukaryotic cells. It is involved in several specialized processes such as protein translation, folding, and transport of proteins to be used in the cell membrane or secreted from the cell. Accumulation of malfolded proteins in the ER activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase, to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA, converting it to a potent UPR transcriptional activator. ER stress also causes the cleavage of ATF6, yielding a cytosolic fragment which migrates to the nucleus and together with XBP-1, activates transcription of UPR-responsive genes. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.;;For images please see PDF data sheet
Description: The substance Cyclopamine is a hedgehog signaling blocker. It is synthetically produced and has a purity of >98%. The pure substance is white solid which is soluble to 5 mM in ethanol.
Description: The substance Cyclopamine is a hedgehog signaling blocker. It is synthetically produced and has a purity of >98%. The pure substance is white solid which is soluble to 5 mM in ethanol.
Description: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GΒL, which stimulates TOR’s kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.;;For images please see PDF data sheet
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 9 binding protein(RGS9BP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 9 binding protein(RGS9BP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 9 binding protein(RGS9BP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 9 binding protein(RGS9BP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 9 binding protein(RGS9BP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The Myc Pathway Reporter kit is designed for monitoring the activity of the Myc signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for c- Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 9 binding protein(RGS9BP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 9 binding protein(RGS9BP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 9 binding protein(RGS9BP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 9 binding protein(RGS9BP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 9 binding protein(RGS9BP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_ The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Description: The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. _x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity. _x000D_
Description: The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.
Description: A competitive ELISA for quantitative measurement of Canine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
Description: The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Description: A competitive ELISA for quantitative measurement of Goat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Delta1 Notch ligand expression lines, Delta2 Jagged1, Jagged2 and receptor Notch1 and Notch2 in the RA group increases. Correlation analysis showed that HSA-miR155-3p is inversely proportional to MALAT1, HSA-miR155-3p is directly proportional to the Notch pathway Delta1, Jagged1, and MALAT1 is inversely proportional to Jagged2. Conclusions In patients with rheumatoid arthritis, lncRNA MALAT1 HSA-mir155-3p reduced and increased, which together regulate Notch signaling pathway changes.
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