Plastic changes in the anterior cingulate cortex (ACC) is very important in hypersensitivity to pain caused by peripheral nerve injury. The Notch signaling pathway has been shown to regulate differentiation and synaptic transmission. Therefore, this study was to investigate the function of Notch signaling pathway in the ACC during nociceptive transmission caused by neuropathy pain
.We adopt Western boltting, N- [N- (3,5-difluorophenacetyl) l-Alanyl] -S- Phenylglycine t-butyl ester (dAPT) microinjections, RNA interference targeting Notch1, hairy and enhancer of split (Hes) 1 or Hes5, electrophysiological recording and behavioral tests to verify the relationship between Notch signaling in the ACC and neuropathic pain in rats adult male Sprague Dawley .Levels of Notch intracellular domain (NICD) increased in the ACC at day 7 after chronic constriction injury (CCI) operation or spared nerve injury (SNI).
Meanwhile, the mRNA level of Notch signaling downstream effector Hes1 Hes5 mRNA increased while the rate unchanged. Injection of DAPT, a γ-Secretase (the key enzyme involved in the Notch pathway) inhibitors, ACC to significantly reversed neuropathic pain behavior. Intra-ACC injection of short hairpin RNA (shRNA) -Notch reduce the expression of NICD and lower potentiation of synaptic transmission in the ACC. In addition, the perception of pain is also addressed in mice undergoing CCI or SNI. This process is mainly mediated by Hes1 downstream effectors, but not Hes5.Based this result, activation of the Notch / Hes1 signaling pathway in the ACC participated in the development of neuropathic pain, suggesting that Notch pathway may be a new therapeutic target to treat chronic pain.
Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain
[Decrease in long chains MALAT1 non-coding RNA expression and increased expression of HSA-miR155-3p involved in the regulation of Notch signaling pathway in patients with rheumatoid arthritis]
Objective To investigate the expression of a long chain of non-coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) and HSA-miR155-3p in patients with rheumatoid arthritis (RA) and their relationship with the Notch signaling pathway, and explore the possibility of RA pathogenesis. Method peripheral blood of patients with RA (RA group) and healthy controls (group NC) was used to screen differentially expressed mRNA by lncRNA and high-throughput gene sequencing.
Reverse-transcription PCR is used to detect the expression of lncRNA MALAT1, HSA-miR155-3p and Notch signaling pathway receptor ligands. Results lncRNA sequencing analysis showed a total of 9158 lncRNAs are expressed differently. Gene ontology (GO) annotation of functional classification revealed that differential mRNA is expressed mainly involved in inflammatory immune response, cellular transcriptional regulation and so on.
path analysis proves that the differentially expressed mRNA was significantly associated with genes involved in rheumatoid arthritis, cellular aging, and the Notch signaling pathway. According to the prediction of cis and trans, Jagged1-2, Delta1-2 and Notch1-2 probably closely related to RA. MALAT1 the RA group was lower than in group NC, and HSA-miR155-3p expression was significantly higher than in the NC group.
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth,
Description: A competitive ELISA for quantitative measurement of Porcine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
ELISA Kit for Pig Suppressor of cytokine signaling 2
Description: A sandwich ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 3 ELISA kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 3 ELISA kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Suppressors Of Cytokine Signaling 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea Pig Suppressors Of Cytokine Signaling 1 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig RGS2/Regulator of G-protein signaling 2 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator Of G Protein Signaling 4 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator Of G Protein Signaling 4 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator Of G Protein Signaling 4 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Guinea pig WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig WNT1 Inducible Signaling Pathway Protein 1 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 17(RGS17) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 17(RGS17) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G protein signaling 20(RGS20) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Regulator of G protein signaling 20(RGS20) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Regulator of G-protein signaling 5 (RGS5) ELISA Kit
Delta1 Notch ligand expression lines, Delta2 Jagged1, Jagged2 and receptor Notch1 and Notch2 in the RA group increases. Correlation analysis showed that HSA-miR155-3p is inversely proportional to MALAT1, HSA-miR155-3p is directly proportional to the Notch pathway Delta1, Jagged1, and MALAT1 is inversely proportional to Jagged2. Conclusions In patients with rheumatoid arthritis, lncRNA MALAT1 HSA-mir155-3p reduced and increased, which together regulate Notch signaling pathway changes.
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Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Equipments | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | quipments | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Uncategorized | Vector & Virus | Western Blot
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