Background: Recently, Notch signaling pathway has gained attention as a potential therapeutic target for chemotherapeutic intervention. However, the efficacy of previously known inhibitors of Notch in colon cancer remains unclear. The purpose of this study was to determine the effect of andrographolide in aberrantly activated Notch signaling in SW-480 cells in vitro.

Methods: The potential cytostatic andrografolida on SW-480 cells was evaluated by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide (MTT) assay, assessment of morphology and colony formation test. FITC apoptosis activity was evaluated by Annexin V assay, 4 ‘, 6-diamidino-2-phenylindole (DAPI), Hoechst, Rhodamine 123 and Mito Tracker staining CMXRos. Scratching assay for assessment of potential migration. 7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA) staining was used to evaluate Reactive Oxygen Species (ROS) generation. mRNA relative expression of Bax, Bcl-2, NOTCH 1 and jagged 1 was estimated by real-time quantitative reverse transcription PCR (qRT-PCR). cell cycle phase distribution was evaluated Annexin V-FITC / PI staining.

Results: MTT assay showed a dose and time dependent cytoxicity of andrographolide on SW-480 cells. It also inhibits migratory and colony forming potential of SW-480 cells. In addition, andrografolida also showed impaired mitochondrial membrane potential and induced apoptosis through nuclear condensation. Flow cytometric evaluation showed improved early and late andrographolide cells induced apoptosis and upregulation of proapoptotic (Bax and Bad) and downregulation of Bcl-2 antiapoptotic in SW-480 treated cells. Andrographolide plus intracellular ROS and induced G0 / G1 phase cell cycle arrest in SW480 colon cancer cells. Furthermore, andrografolida suppress Notch signaling by reducing the expression of NOTCH 1 and jagged one.
Conclusions: Our findings indicate that the andrographolide constraints SW-480 cell growth through inhibition of the Notch signaling pathway.

MicroRNA-133b exacerbate atherosclerosis by activating the Notch signaling pathway1

The purpose of this study was to determine the effect of miR-133b in atherosclerosis (AS). A rat model of AS (AS Group) was established, and serum levels of total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein (LDL) cholesterol were detected. The thoracic aorta tissue was subjected to hematoxylin and eosin staining for pathological examination. Mice were intravenously injected with microRNA (mir) -133b mimic (group miR-133b mimic + US) and miR-133b mimic negative control (miR-133b group NC + US).

Normal mice given the name of Sham group. reconstruction parameters of vascular, inflammatory factors Ratio Collagen / Vascular Area (CA / CVA) and serum of mice in each group were detected. MRNA expression was measured by quantitative reverse transcription-PCR and protein expression was determined by western blot analysis. An in vitro model of the US induced in vascular smooth muscle cells (VSMC) using oxidized (ox) -LDL. CCK-8 and wound healing tests used to detect cell proliferation and migration.

Compared with Sham group, the rats of the US group, AS + miR-133b mimic NC group and the group AS + miR-133b have a higher intima thickness (IT), tumor necrosis factor (TNF) -α and monocytes kemoatraktan protein (MCP) -1 level, as well as increased expression of Notch1 and Jagged1; and they have a lower medial thickness (MT), the ratio CA / CVA and Notch3 expression (all P <0.05).

iFluor™ 610 succinimidyl ester
1038	AAT Bioquest	1 mg	EUR 219

iFluor™ A7 SE
1039	AAT Bioquest	1 mg	EUR 219

iFluor™ 546 succinimidyl ester
1048	AAT Bioquest	1 mg	EUR 219

iFluor™ 568 succinimidyl ester
1049	AAT Bioquest	1 mg	EUR 219

iFluor™ 450 maleimide
1057	AAT Bioquest	1 mg	EUR 219

iFluor™ 647 amine
1074	AAT Bioquest	1 mg	EUR 219

iFluor™ 680 amine
1076	AAT Bioquest	1 mg	EUR 219

iFluor™ 700 amine
1077	AAT Bioquest	1 mg	EUR 219

iFluor™ 710 amine
1078	AAT Bioquest	1 mg	EUR 219

iFluor™ 750 amine
1079	AAT Bioquest	1 mg	EUR 219

iFluor™ 680 hydrazide
1086	AAT Bioquest	1 mg	EUR 219

iFluor™ 700 hydrazide
1087	AAT Bioquest	1 mg	EUR 219

iFluor™ 750 hydrazide
1088	AAT Bioquest	1 mg	EUR 219

ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11001	AAT Bioquest	1 L	EUR 202

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11003	AAT Bioquest	1 L	EUR 480

ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
11004	AAT Bioquest	5x10 mL	EUR 132

Amplite™ Blue
11005	AAT Bioquest	25 mg	EUR 176

Amplite™ IR
11009	AAT Bioquest	1 mg	EUR 132

Amplite™ Red
11011	AAT Bioquest	1000 Assays	EUR 132

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11012	AAT Bioquest	100 ml	EUR 115

ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1105	AAT Bioquest	2 Labelings	EUR 176

Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*
11050	AAT Bioquest	1 g	EUR 115

iFluor™ 488 tyramide
11060	AAT Bioquest	1 mg	EUR 219

Azido-Cy5 tyramide
11061	AAT Bioquest	1 mg	EUR 306

Amplite™ Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
11100	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
11105	AAT Bioquest	500 tests	EUR 219

ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1114	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1120	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1123	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1126	AAT Bioquest	2 Labelings	EUR 50

Amplite™ Colorimetric Glucose Oxidase Assay Kit
11299	AAT Bioquest	500 Tests	EUR 306

ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1130	AAT Bioquest	2 Labelings	EUR 176

Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
11300	AAT Bioquest	500 Tests	EUR 219

Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*
11301	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*
11302	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
11303	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*
11304	AAT Bioquest	200 Tests	EUR 219

Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit
11305	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence*
11306	AAT Bioquest	200 Tests	EUR 219

Amplite™ Colorimetric Xanthine Oxidase Assay Kit
11307	AAT Bioquest	200 Tests	EUR 219

mFluor™ Violet 450 acid
1140	AAT Bioquest	5 mg	EUR 219

Amplite™ Colorimetric Acetylcholinesterase Assay Kit
11400	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
11401	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*
11402	AAT Bioquest	200 Tests	EUR 219

Amplite™ Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
11403	AAT Bioquest	200 Tests	EUR 219

mFluor™ Violet 510 acid
1141	AAT Bioquest	5 mg	EUR 306

mFluor™ Violet 540 acid
1142	AAT Bioquest	5 mg	EUR 306

mFluor™ Blue 570 acid
1143	AAT Bioquest	5 mg	EUR 306

mFluor™ Green 620 acid
1144	AAT Bioquest	5 mg	EUR 306

mFluor™ Yellow 630 acid
1145	AAT Bioquest	5 mg	EUR 50

mFluor™ Red 700 acid
1146	AAT Bioquest	5 mg	EUR 306

mFluor™ Violet 500 SE
1149	AAT Bioquest	1 mg	EUR 219

mFluor™ Violet 450 SE
1150	AAT Bioquest	1 mg	EUR 219

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*
11503	AAT Bioquest	200 Tests	EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*
11504	AAT Bioquest	100 Tests	EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*
11505	AAT Bioquest	100 Tests	EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
11506	AAT Bioquest	100 Tests	EUR 263

mFluor™ Violet 510 SE
1151	AAT Bioquest	1 mg	EUR 219

mFluor™ Violet 540 SE
1152	AAT Bioquest	1 mg	EUR 219

Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
11540	AAT Bioquest	1000 Tests	EUR 219

Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
11541	AAT Bioquest	1000 Tests	EUR 219

Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
11551	AAT Bioquest	500 Tests	EUR 219

Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
11552	AAT Bioquest	500 Tests	EUR 219

Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*
11553	AAT Bioquest	500 Tests	EUR 306

Amplite™ Luminometric Peroxidase (HRP) Assay Kit
11559	AAT Bioquest	500 Tests	EUR 219

Amplite™ Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
11560	AAT Bioquest	200 Tests	EUR 306

mFluor™ Blue 570 SE
1160	AAT Bioquest	1 mg	EUR 306

FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*
11600	AAT Bioquest	5 mg	EUR 132

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*
11610	AAT Bioquest	25 mg	EUR 115

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*
11612	AAT Bioquest	10 g	EUR 898

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*
11614	AAT Bioquest	25 mg	EUR 115

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*
11617	AAT Bioquest	5 g	EUR 480

pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9*
11619	AAT Bioquest	25 mg	EUR 115

CF-MUP, sodium salt *Superior alternative to MUP*
11628	AAT Bioquest	10 mg	EUR 50

mFluor™ Green 620 SE
1165	AAT Bioquest	1 mg	EUR 306

mFluor™ Yellow 630 SE
1170	AAT Bioquest	1 mg	EUR 50

ADP-ribose-pNP
11700	AAT Bioquest	1 mg	EUR 219

mFluor™ Red 780 SE
1191	AAT Bioquest	1 mg	EUR 306

Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
11950	AAT Bioquest	500 Tests	EUR 176

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*
11952	AAT Bioquest	500 Tests	EUR 219

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*
11953	AAT Bioquest	500 Tests	EUR 219

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence*
11954	AAT Bioquest	500 Tests	EUR 219

Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
11956	AAT Bioquest	100 Tests	EUR 219

ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1227	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1235	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1240	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1245	AAT Bioquest	2 Labelings	EUR 176

Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]
12481	AAT Bioquest	25 mg	EUR 219

Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]
12482	AAT Bioquest	100 mg	EUR 480

ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1250	AAT Bioquest	2 Labelings	EUR 176

D-Luciferin, free acid *CAS#: 2591-17-5*
12501	AAT Bioquest	25 mg	EUR 115

D-Luciferin, free acid *CAS#: 2591-17-5*
12502	AAT Bioquest	100 mg	EUR 132

D-Luciferin, free acid *CAS#: 2591-17-5*
12503	AAT Bioquest	1 g	EUR 528

D-Luciferin, potassium salt *CAS#: 115144-35-9*
12505	AAT Bioquest	25 mg	EUR 115

D-Luciferin, potassium salt *CAS#: 115144-35-9*
12506	AAT Bioquest	100 mg	EUR 132

D-Luciferin, potassium salt *CAS#: 115144-35-9*
12507	AAT Bioquest	1 g	EUR 263

D-Luciferin, sodium salt *CAS#: 103404-75-7*
12509	AAT Bioquest	25 mg	EUR 115

D-Luciferin, sodium salt *CAS#: 103404-75-7*
12510	AAT Bioquest	100 mg	EUR 132

D-Luciferin, sodium salt *CAS#: 103404-75-7*
12511	AAT Bioquest	1 g	EUR 528

D-Luciferin phosphate *CAS 145613-12-3*
12512	AAT Bioquest	1 mg	EUR 219

D-Luciferin galactoside *CAS 131474-38-9*
12513	AAT Bioquest	5 mg	EUR 219

D-Luciferin methyl ester *CAS 73918-26-0*
12514	AAT Bioquest	5 mg	EUR 219

D-Luciferin ethyl ester *CAS 135251-85-3*
12515	AAT Bioquest	5 mg	EUR 219

D-Luciferin acetate
12516	AAT Bioquest	5 mg	EUR 219

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12518	AAT Bioquest	1 plate	EUR 176

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12519	AAT Bioquest	10 plates	EUR 393

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12520	AAT Bioquest	100 plates	EUR 2616

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12530	AAT Bioquest	1 plate	EUR 219

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12531	AAT Bioquest	10 Plates	EUR 702

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12532	AAT Bioquest	100 plates	EUR 3486

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12535	AAT Bioquest	1 plate	EUR 219

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12536	AAT Bioquest	10 plates	EUR 876

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12537	AAT Bioquest	100 plates	EUR 3486

ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1255	AAT Bioquest	2 Labelings	EUR 176

Amplite™ Colorimetric Beta-Lactamase Activity Assay Kit
12551	AAT Bioquest	200 Tests	EUR 393

BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]
12560	AAT Bioquest	1 mg	EUR 219

BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]
12561	AAT Bioquest	5 mg	EUR 393

ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1260	AAT Bioquest	2 Labelings	EUR 176

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
12601	AAT Bioquest	500 Tests	EUR 306

Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
12602	AAT Bioquest	200 Tests	EUR 306

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*
12603	AAT Bioquest	200 Tests	EUR 306

Amplite™ Colorimetric Beta-Galactosidase Assay Kit
12604	AAT Bioquest	200 Tests	EUR 306

HIS Lite™ Cy3 Bis NTA-Ni Complex
12610	AAT Bioquest	1 mg	EUR 393

HIS Lite™ Cy5 Bis NTA-Ni Complex
12613	AAT Bioquest	1 mg	EUR 393

ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1265	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1290	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1292	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1294	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1299	AAT Bioquest	2 Labelings	EUR 176

ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*
1300	AAT Bioquest	2 Labelings	EUR 876

Benzoyl-Leu-Gly-Arg-Aminoluciferin
13000	AAT Bioquest	1 mg	EUR 480

BOC-Leu-Gly-Arg-Aminoluciferin
13001	AAT Bioquest	1 mg	EUR 480

BOC-Val-Pro-Arg-Aminoluciferin
13002	AAT Bioquest	1 mg	EUR 480

D-VLK-AMC [D-Val-Leu-Lys-AMC]
13201	AAT Bioquest	5 mg	EUR 132

Ac-DEVD-AFC *CAS 201608-14-2*
13401	AAT Bioquest	5 mg	EUR 132

Ac-DEVD-AMC *CAS 169332-61-0*
13402	AAT Bioquest	5 mg	EUR 132

Ac-DEVD-CHO *CAS 169332-60-9*
13403	AAT Bioquest	1 mg	EUR 132

Ac-DEVD-pNA *CAS 189950-66-1*
13405	AAT Bioquest	5 mg	EUR 132

Ac-IETD-AFC *CAS 211990-57-7*
13410	AAT Bioquest	5 mg	EUR 219

Ac-IETD-AMC
13411	AAT Bioquest	5 mg	EUR 219

Ac-IETD-CHO *CAS 191338-86-0*
13412	AAT Bioquest	5 mg	EUR 219

Z-IETD-pNA *CAS 219138-21-3*
13413	AAT Bioquest	5 mg	EUR 219

D-Aminoluciferin
13415	AAT Bioquest	10 mg	EUR 219

Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*
1342	AAT Bioquest	2 Labelings	EUR 306

Z-DEVD-AMC
13421	AAT Bioquest	5 mg	EUR 176

Z-IETD-AFC *CAS 219138-02-0*
13425	AAT Bioquest	5 mg	EUR 158

Ac-LEHD-AMC *CAS 292633-16-0*
13426	AAT Bioquest	5 mg	EUR 176

(Ac-LEHD)2-R110
13427	AAT Bioquest	1 mg	EUR 132

(Ac-IETD)2-R110
13431	AAT Bioquest	1 mg	EUR 132

Z-DEVD-ProRed™ 620
13433	AAT Bioquest	1 mg	EUR 306

Z-IETD-ProRed™ 620
13434	AAT Bioquest	1 mg	EUR 306

Z-LEHD-ProRed™ 620
13435	AAT Bioquest	1 mg	EUR 306

Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8*
13445	AAT Bioquest	10 mg	EUR 176

Gly-Pro-AMC *CAS 115035-46-6*
13450	AAT Bioquest	5 mg	EUR 132

In addition, miR-133b mimic promoted proliferation and migration, Notch1 and Jagged1 upregulated and downregulated Notch3 in VSMC ox-LDL-induced. Taken together, miR-133b worsen the US by activating Notch signaling pathway, which could serve as a potential target for the treatment of AS.