Background: Recently, Notch signaling pathway has gained attention as a potential therapeutic target for chemotherapeutic intervention. However, the efficacy of previously known inhibitors of Notch in colon cancer remains unclear. The purpose of this study was to determine the effect of andrographolide in aberrantly activated Notch signaling in SW-480 cells in vitro.

Methods: The potential cytostatic andrografolida on SW-480 cells was evaluated by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide (MTT) assay, assessment of morphology and colony formation test. FITC apoptosis activity was evaluated by Annexin V assay, 4 ‘, 6-diamidino-2-phenylindole (DAPI), Hoechst, Rhodamine 123 and Mito Tracker staining CMXRos. Scratching assay for assessment of potential migration. 7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA) staining was used to evaluate Reactive Oxygen Species (ROS) generation. mRNA relative expression of Bax, Bcl-2, NOTCH 1 and jagged 1 was estimated by real-time quantitative reverse transcription PCR (qRT-PCR). cell cycle phase distribution was evaluated Annexin V-FITC / PI staining.

Results: MTT assay showed a dose and time dependent cytoxicity of andrographolide on SW-480 cells. It also inhibits migratory and colony forming potential of SW-480 cells. In addition, andrografolida also showed impaired mitochondrial membrane potential and induced apoptosis through nuclear condensation. Flow cytometric evaluation showed improved early and late andrographolide cells induced apoptosis and upregulation of proapoptotic (Bax and Bad) and downregulation of Bcl-2 antiapoptotic in SW-480 treated cells. Andrographolide plus intracellular ROS and induced G0 / G1 phase cell cycle arrest in SW480 colon cancer cells. Furthermore, andrografolida suppress Notch signaling by reducing the expression of NOTCH 1 and jagged one.
Conclusions: Our findings indicate that the andrographolide constraints SW-480 cell growth through inhibition of the Notch signaling pathway.

MicroRNA-133b exacerbate atherosclerosis by activating the Notch signaling pathway1

The purpose of this study was to determine the effect of miR-133b in atherosclerosis (AS). A rat model of AS (AS Group) was established, and serum levels of total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein (LDL) cholesterol were detected. The thoracic aorta tissue was subjected to hematoxylin and eosin staining for pathological examination. Mice were intravenously injected with microRNA (mir) -133b mimic (group miR-133b mimic + US) and miR-133b mimic negative control (miR-133b group NC + US).

Normal mice given the name of Sham group. reconstruction parameters of vascular, inflammatory factors Ratio Collagen / Vascular Area (CA / CVA) and serum of mice in each group were detected. MRNA expression was measured by quantitative reverse transcription-PCR and protein expression was determined by western blot analysis. An in vitro model of the US induced in vascular smooth muscle cells (VSMC) using oxidized (ox) -LDL. CCK-8 and wound healing tests used to detect cell proliferation and migration.

Compared with Sham group, the rats of the US group, AS + miR-133b mimic NC group and the group AS + miR-133b have a higher intima thickness (IT), tumor necrosis factor (TNF) -α and monocytes kemoatraktan protein (MCP) -1 level, as well as increased expression of Notch1 and Jagged1; and they have a lower medial thickness (MT), the ratio CA / CVA and Notch3 expression (all P <0.05).

ETHYL VIOLET AZIDE BROTH
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ENDO AGAR
E05-110-10kg	Alphabiosciences	10 kg	EUR 2381

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E05-110-2Kg	Alphabiosciences	2 Kg	EUR 557

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E05-110-500g	Alphabiosciences	500 g	EUR 188

APT AGAR
A01-101-10kg	Alphabiosciences	10 kg	EUR 1173

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AGAR, BACTERIOLOGICAL
A01-102-10kg	Alphabiosciences	10 kg	EUR 1684

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A01-102-2kg	Alphabiosciences	2kg	EUR 405

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A01-102-500g	Alphabiosciences	500 g	EUR 146

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A01-102IHG-10kg	Alphabiosciences	10 kg	EUR 2219

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A01-102IHG-2Kg	Alphabiosciences	2 Kg	EUR 521

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A01-102IHG-500g	Alphabiosciences	500 g	EUR 178

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A01-102N-10kg	Alphabiosciences	10 kg	EUR 3910

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A01-102N-2kg	Alphabiosciences	2kg	EUR 889

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A01-102N-500g	Alphabiosciences	500 g	EUR 278

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AZIDE BLOOD AGAR BASE
A01-113-10kg	Alphabiosciences	10 kg	EUR 2532

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A01-113-2kg	Alphabiosciences	2kg	EUR 589

AZIDE BLOOD AGAR BASE
A01-113-500g	Alphabiosciences	500 g	EUR 197

AZIDE DEXTROSE BROTH
A01-114-10kg	Alphabiosciences	10 kg	EUR 868

AZIDE DEXTROSE BROTH
A01-114-2Kg	Alphabiosciences	2 Kg	EUR 228

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A01-114-500g	Alphabiosciences	500 g	EUR 98

ANAEROBIC AGAR
A01-115-10kg	Alphabiosciences	10 kg	EUR 2556

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A01-115-2kg	Alphabiosciences	2kg	EUR 595

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A01-115-500g	Alphabiosciences	500 g	EUR 198

A-1 MEDIUM
A01-116-10kg	Alphabiosciences	10 kg	EUR 1034

A-1 MEDIUM
A01-116-2Kg	Alphabiosciences	2 Kg	EUR 264

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A01-116-500g	Alphabiosciences	500 g	EUR 108

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A01-118-500g	Alphabiosciences	500 g	EUR 494

IL-2 Receptor ?, Human Recombinant
7100-10	Biovision		EUR 196

IL-2 Receptor ?, Human Recombinant
7100-50	Biovision		EUR 637

Human CellExp? IL-4 R?, Human Recombinant
7101-10	Biovision		EUR 229

Human CellExp? IL-4 R?, Human Recombinant
7101-50	Biovision		EUR 881

Human CellExp? IL-6 R?, Human Recombinant
7102-10	Biovision		EUR 207

Human CellExp? IL-6 R?, Human Recombinant
7102-50	Biovision		EUR 800

IL-9, Rat Recombinant
7103-10	Biovision		EUR 278

IL-9, Rat Recombinant
7103-50	Biovision		EUR 1132

IL-12p80, Human Recombinant
7104-10	Biovision		EUR 392

IL-12p80, Human Recombinant
7104-50	Biovision		EUR 1675

IL-17A, Mouse Recombinant
7105-10	Biovision		EUR 196

IL-17A, Mouse Recombinant
7105-50	Biovision		EUR 675

IL-17B, Human Recombinant
7106-10	Biovision		EUR 196

IL-17B, Human Recombinant
7106-50	Biovision		EUR 675

IL-17D, Human Recombinant
7107-10	Biovision		EUR 196

IL-17D, Human Recombinant
7107-50	Biovision		EUR 675

Human CellExp? IL-34, Human Recombinant
7108-10	Biovision		EUR 278

Human CellExp? IL-34, Human Recombinant
7108-50	Biovision		EUR 1132

IL-36RA, Human Recombinant
7109-10	Biovision		EUR 196

IL-36RA, Human Recombinant
7109-50	Biovision		EUR 675

IL-36?, Human Recombinant
7110-10	Biovision		EUR 278

IL-36?, Human Recombinant
7110-50	Biovision		EUR 1132

IL-36?, Human Recombinant
7111-10	Biovision		EUR 278

IL-36?, Human Recombinant
7111-50	Biovision		EUR 1132

Activin B, Human Recombinant
7112-10	Biovision		EUR 479

Activin B, Human Recombinant
7112-50	Biovision		EUR 2094

AITRL, Human Recombinant
7113-10	Biovision		EUR 251

AITRL, Human Recombinant
7113-50	Biovision		EUR 936

Amphiregulin, Human Recombinant
7114-10	Biovision		EUR 147

Amphiregulin, Human Recombinant
7114-50	Biovision		EUR 376

ANG-1, Human Recombinant
7115-10	Biovision		EUR 251

ANG-1, Human Recombinant
7115-50	Biovision		EUR 936

ANG-2, Human Recombinant
7116-10	Biovision		EUR 251

ANG-2, Human Recombinant
7116-50	Biovision		EUR 936

APRIL, Human Recombinant
7117-10	Biovision		EUR 311

APRIL, Human Recombinant
7117-50	Biovision		EUR 1300

APRIL, Mouse Recombinant
7118-10	Biovision		EUR 196

APRIL, Mouse Recombinant
7118-50	Biovision		EUR 675

Betacellulin, Murine Recombinant
7119-10	Biovision		EUR 196

Betacellulin, Murine Recombinant
7119-50	Biovision		EUR 675

Cardiotrophin-1, Murine Recombinant
7121-10	Biovision		EUR 278

Cardiotrophin-1, Murine Recombinant
7121-50	Biovision		EUR 1132

sCD100/Semaphorin-4D, Human Recombinant
7126-10	Biovision		EUR 251

sCD100/Semaphorin-4D, Human Recombinant
7126-50	Biovision		EUR 936

CTGFL/WISP-2, Human Recombinant
7129-10	Biovision		EUR 234

CTGFL/WISP-2, Human Recombinant
7129-50	Biovision		EUR 860

Human CellExp? DKK-1, Human Recombinant
7132-10	Biovision		EUR 278

Human CellExp? DKK-1, Human Recombinant
7132-50	Biovision		EUR 1132

Human CellExp? sDLL-1, Human Recombinant
7133-10	Biovision		EUR 207

Human CellExp? sDLL-1, Human Recombinant
7133-50	Biovision		EUR 675

Human CellExp? sDLL-4, Human Recombinant
7134-10	Biovision		EUR 207

Human CellExp? sDLL-4, Human Recombinant
7134-50	Biovision		EUR 675

Eotaxin-2/CCL24, murine recombinant
7137-10	Biovision		EUR 207

Eotaxin-2/CCL24, murine recombinant
7137-50	Biovision		EUR 675

Eotaxin-3/CCL26, human recombinant
7138-10	Biovision		EUR 207

Eotaxin-3/CCL26, human recombinant
7138-50	Biovision		EUR 675

Human CellExp? Fetuin A/ASHG, Human Recombinant
7142-10	Biovision		EUR 153

Human CellExp? Fetuin A/ASHG, Human Recombinant
7142-50	Biovision		EUR 381

Histone H3 Peptide, biotin conjugate
7143-01	Biovision		EUR 240

Histone H3 Peptide, biotin conjugate
7143-1	Biovision		EUR 1132

Histone H4 Peptide, tetra-acetylated, biotin conjugate
7144-01	Biovision		EUR 240

Histone H4 Peptide, tetra-acetylated, biotin conjugate
7144-1	Biovision		EUR 1132

FGF-5, human recombinant
7147-10	Biovision		EUR 142

FGF-5, human recombinant
7147-50	Biovision		EUR 359

FGF-6, human recombinant
7148-10	Biovision		EUR 207

FGF-6, human recombinant
7148-50	Biovision		EUR 675

FGF-16, human recombinant
7149-10	Biovision		EUR 207

FGF-16, human recombinant
7149-50	Biovision		EUR 675

FGF-17, human recombinant
7150-10	Biovision		EUR 207

FGF-17, human recombinant
7150-50	Biovision		EUR 675

FGF-23, human recombinant
7151-10	Biovision		EUR 229

FGF-23, human recombinant
7151-50	Biovision		EUR 860

GASP-1, human recombinant
7152-10	Biovision		EUR 229

GASP-1, human recombinant
7152-50	Biovision		EUR 833

GCP-2 (CXCL6), human recombinant
7153-10	Biovision		EUR 207

GCP-2 (CXCL6), human recombinant
7153-50	Biovision		EUR 675

BMP-9/GDF-2, human recombinant
7154-10	Biovision		EUR 278

BMP-9/GDF-2, human recombinant
7154-50	Biovision		EUR 1132

BMP-11/GDF-11, human recombinant
7155-10	Biovision		EUR 240

BMP-11/GDF-11, human recombinant
7155-50	Biovision		EUR 860

Gremlin-1, human recombinant
7159-10	Biovision		EUR 142

Gremlin-1, human recombinant
7159-50	Biovision		EUR 359

ICAM-1, human recombinant
7161-10	Biovision		EUR 142

ICAM-1, human recombinant
7161-50	Biovision		EUR 359

IFN-?1, human recombinant
7162-10	Biovision		EUR 207

IFN-?1, human recombinant
7162-50	Biovision		EUR 735

IFN-?2, human recombinant
7163-10	Biovision		EUR 207

IFN-?2, human recombinant
7163-50	Biovision		EUR 675

IGF-BP2, human recombinant
7165-10	Biovision		EUR 251

IGF-BP2, human recombinant
7165-50	Biovision		EUR 936

IGF-BP4, human recombinant
7166-10	Biovision		EUR 251

IGF-BP4, human recombinant
7166-50	Biovision		EUR 936

IGF-BP7, human recombinant
7167-10	Biovision		EUR 207

IGF-BP7, human recombinant
7167-50	Biovision		EUR 675

MCP-5/CCL12, murine recombinant
7168-10	Biovision		EUR 207

MCP-5/CCL12, murine recombinant
7168-50	Biovision		EUR 675

MIP-1?/CCL3, human recombinant
7173-10	Biovision		EUR 207

MIP-1?/CCL3, human recombinant
7173-50	Biovision		EUR 675

MIP-1?/CCL9/10, murine recombinant
7174-10	Biovision		EUR 207

MIP-1?/CCL9/10, murine recombinant
7174-50	Biovision		EUR 675

MIP-3/CCL23, human recombinant
7175-10	Biovision		EUR 207

MIP-3/CCL23, human recombinant
7175-50	Biovision		EUR 675

Nesfatin-1, human recombinant
7178-10	Biovision		EUR 137

Nesfatin-1, human recombinant
7178-50	Biovision		EUR 272

Human CellExp? PECAM-1, Human Recombinant
7184-10	Biovision		EUR 153

Human CellExp? PECAM-1, Human Recombinant
7184-50	Biovision		EUR 381

PF-4/CXCL4, murine recombinant
7187-10	Biovision		EUR 207

PF-4/CXCL4, murine recombinant
7187-50	Biovision		EUR 675

R-Spondin-1, human recombinant
7189-10	Biovision		EUR 207

R-Spondin-1, human recombinant
7189-50	Biovision		EUR 675

R-Spondin-2, human recombinant
7190-10	Biovision		EUR 207

In addition, miR-133b mimic promoted proliferation and migration, Notch1 and Jagged1 upregulated and downregulated Notch3 in VSMC ox-LDL-induced. Taken together, miR-133b worsen the US by activating Notch signaling pathway, which could serve as a potential target for the treatment of AS.