Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

Background: Recently, Notch signaling pathway has gained attention as a potential therapeutic target for chemotherapeutic intervention. However, the efficacy of previously known inhibitors of Notch in colon cancer remains unclear. The purpose of this study was to determine the effect of andrographolide in aberrantly activated Notch signaling in SW-480 cells in vitro.


Methods: The potential cytostatic andrografolida on SW-480 cells was evaluated by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide (MTT) assay, assessment of morphology and colony formation test. FITC apoptosis activity was evaluated by Annexin V assay, 4 ‘, 6-diamidino-2-phenylindole (DAPI), Hoechst, Rhodamine 123 and Mito Tracker staining CMXRos. Scratching assay for assessment of potential migration. 7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA) staining was used to evaluate Reactive Oxygen Species (ROS) generation. mRNA relative expression of Bax, Bcl-2, NOTCH 1 and jagged 1 was estimated by real-time quantitative reverse transcription PCR (qRT-PCR). cell cycle phase distribution was evaluated Annexin V-FITC / PI staining.


Results: MTT assay showed a dose and time dependent cytoxicity of andrographolide on SW-480 cells. It also inhibits migratory and colony forming potential of SW-480 cells. In addition, andrografolida also showed impaired mitochondrial membrane potential and induced apoptosis through nuclear condensation. Flow cytometric evaluation showed improved early and late andrographolide cells induced apoptosis and upregulation of proapoptotic (Bax and Bad) and downregulation of Bcl-2 antiapoptotic in SW-480 treated cells. Andrographolide plus intracellular ROS and induced G0 / G1 phase cell cycle arrest in SW480 colon cancer cells. Furthermore, andrografolida suppress Notch signaling by reducing the expression of NOTCH 1 and jagged one.
Conclusions: Our findings indicate that the andrographolide constraints SW-480 cell growth through inhibition of the Notch signaling pathway.

Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway
Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

MicroRNA-133b exacerbate atherosclerosis by activating the Notch signaling pathway1

The purpose of this study was to determine the effect of miR-133b in atherosclerosis (AS). A rat model of AS (AS Group) was established, and serum levels of total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein (LDL) cholesterol were detected. The thoracic aorta tissue was subjected to hematoxylin and eosin staining for pathological examination. Mice were intravenously injected with microRNA (mir) -133b mimic (group miR-133b mimic + US) and miR-133b mimic negative control (miR-133b group NC + US).

Normal mice given the name of Sham group. reconstruction parameters of vascular, inflammatory factors Ratio Collagen / Vascular Area (CA / CVA) and serum of mice in each group were detected. MRNA expression was measured by quantitative reverse transcription-PCR and protein expression was determined by western blot analysis. An in vitro model of the US induced in vascular smooth muscle cells (VSMC) using oxidized (ox) -LDL. CCK-8 and wound healing tests used to detect cell proliferation and migration.

Compared with Sham group, the rats of the US group, AS + miR-133b mimic NC group and the group AS + miR-133b have a higher intima thickness (IT), tumor necrosis factor (TNF) -α and monocytes kemoatraktan protein (MCP) -1 level, as well as increased expression of Notch1 and Jagged1; and they have a lower medial thickness (MT), the ratio CA / CVA and Notch3 expression (all P <0.05).

ETHYL VIOLET AZIDE BROTH

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APT AGAR

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AGAR, BACTERIOLOGICAL

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EUR 1684

AGAR, BACTERIOLOGICAL

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EUR 405

AGAR, BACTERIOLOGICAL

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EUR 146

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EUR 2219

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EUR 197

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EUR 868

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EUR 98

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EUR 2556

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EUR 1034

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7100-10
EUR 196

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7100-50
EUR 637

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7101-10
EUR 229

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7101-50
EUR 881

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7102-10
EUR 207

Human CellExp? IL-6 R?, Human Recombinant

7102-50
EUR 800

IL-9, Rat Recombinant

7103-10
EUR 278

IL-9, Rat Recombinant

7103-50
EUR 1132

IL-12p80, Human Recombinant

7104-10
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7104-50
EUR 1675

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7105-10
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EUR 675

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IL-17B, Human Recombinant

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IL-17D, Human Recombinant

7107-10
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IL-17D, Human Recombinant

7107-50
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Human CellExp? IL-34, Human Recombinant

7108-10
EUR 278

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7108-50
EUR 1132

IL-36RA, Human Recombinant

7109-10
EUR 196

IL-36RA, Human Recombinant

7109-50
EUR 675

IL-36?, Human Recombinant

7110-10
EUR 278

IL-36?, Human Recombinant

7110-50
EUR 1132

IL-36?, Human Recombinant

7111-10
EUR 278

IL-36?, Human Recombinant

7111-50
EUR 1132

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7112-10
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EUR 936

CTGFL/WISP-2, Human Recombinant

7129-10
EUR 234

CTGFL/WISP-2, Human Recombinant

7129-50
EUR 860

Human CellExp? DKK-1, Human Recombinant

7132-10
EUR 278

Human CellExp? DKK-1, Human Recombinant

7132-50
EUR 1132

Human CellExp? sDLL-1, Human Recombinant

7133-10
EUR 207

Human CellExp? sDLL-1, Human Recombinant

7133-50
EUR 675

Human CellExp? sDLL-4, Human Recombinant

7134-10
EUR 207

Human CellExp? sDLL-4, Human Recombinant

7134-50
EUR 675

Eotaxin-2/CCL24, murine recombinant

7137-10
EUR 207

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7137-50
EUR 675

Eotaxin-3/CCL26, human recombinant

7138-10
EUR 207

Eotaxin-3/CCL26, human recombinant

7138-50
EUR 675

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7142-10
EUR 153

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7142-50
EUR 381

Histone H3 Peptide, biotin conjugate

7143-01
EUR 240

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7143-1
EUR 1132

Histone H4 Peptide, tetra-acetylated, biotin conjugate

7144-01
EUR 240

Histone H4 Peptide, tetra-acetylated, biotin conjugate

7144-1
EUR 1132

FGF-5, human recombinant

7147-10
EUR 142

FGF-5, human recombinant

7147-50
EUR 359

FGF-6, human recombinant

7148-10
EUR 207

FGF-6, human recombinant

7148-50
EUR 675

FGF-16, human recombinant

7149-10
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FGF-16, human recombinant

7149-50
EUR 675

FGF-17, human recombinant

7150-10
EUR 207

FGF-17, human recombinant

7150-50
EUR 675

FGF-23, human recombinant

7151-10
EUR 229

FGF-23, human recombinant

7151-50
EUR 860

GASP-1, human recombinant

7152-10
EUR 229

GASP-1, human recombinant

7152-50
EUR 833

GCP-2 (CXCL6), human recombinant

7153-10
EUR 207

GCP-2 (CXCL6), human recombinant

7153-50
EUR 675

BMP-9/GDF-2, human recombinant

7154-10
EUR 278

BMP-9/GDF-2, human recombinant

7154-50
EUR 1132

BMP-11/GDF-11, human recombinant

7155-10
EUR 240

BMP-11/GDF-11, human recombinant

7155-50
EUR 860

Gremlin-1, human recombinant

7159-10
EUR 142

Gremlin-1, human recombinant

7159-50
EUR 359

ICAM-1, human recombinant

7161-10
EUR 142

ICAM-1, human recombinant

7161-50
EUR 359

IFN-?1, human recombinant

7162-10
EUR 207

IFN-?1, human recombinant

7162-50
EUR 735

IFN-?2, human recombinant

7163-10
EUR 207

IFN-?2, human recombinant

7163-50
EUR 675

IGF-BP2, human recombinant

7165-10
EUR 251

IGF-BP2, human recombinant

7165-50
EUR 936

IGF-BP4, human recombinant

7166-10
EUR 251

IGF-BP4, human recombinant

7166-50
EUR 936

IGF-BP7, human recombinant

7167-10
EUR 207

IGF-BP7, human recombinant

7167-50
EUR 675

MCP-5/CCL12, murine recombinant

7168-10
EUR 207

MCP-5/CCL12, murine recombinant

7168-50
EUR 675

MIP-1?/CCL3, human recombinant

7173-10
EUR 207

MIP-1?/CCL3, human recombinant

7173-50
EUR 675

MIP-1?/CCL9/10, murine recombinant

7174-10
EUR 207

MIP-1?/CCL9/10, murine recombinant

7174-50
EUR 675

MIP-3/CCL23, human recombinant

7175-10
EUR 207

MIP-3/CCL23, human recombinant

7175-50
EUR 675

Nesfatin-1, human recombinant

7178-10
EUR 137

Nesfatin-1, human recombinant

7178-50
EUR 272

Human CellExp? PECAM-1, Human Recombinant

7184-10
EUR 153

Human CellExp? PECAM-1, Human Recombinant

7184-50
EUR 381

PF-4/CXCL4, murine recombinant

7187-10
EUR 207

PF-4/CXCL4, murine recombinant

7187-50
EUR 675

R-Spondin-1, human recombinant

7189-10
EUR 207

R-Spondin-1, human recombinant

7189-50
EUR 675

R-Spondin-2, human recombinant

7190-10
EUR 207

In addition, miR-133b mimic promoted proliferation and migration, Notch1 and Jagged1 upregulated and downregulated Notch3 in VSMC ox-LDL-induced. Taken together, miR-133b worsen the US by activating Notch signaling pathway, which could serve as a potential target for the treatment of AS.

Anthony

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