Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

Background: Recently, Notch signaling pathway has gained attention as a potential therapeutic target for chemotherapeutic intervention. However, the efficacy of previously known inhibitors of Notch in colon cancer remains unclear. The purpose of this study was to determine the effect of andrographolide in aberrantly activated Notch signaling in SW-480 cells in vitro.


Methods: The potential cytostatic andrografolida on SW-480 cells was evaluated by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide (MTT) assay, assessment of morphology and colony formation test. FITC apoptosis activity was evaluated by Annexin V assay, 4 ‘, 6-diamidino-2-phenylindole (DAPI), Hoechst, Rhodamine 123 and Mito Tracker staining CMXRos. Scratching assay for assessment of potential migration. 7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA) staining was used to evaluate Reactive Oxygen Species (ROS) generation. mRNA relative expression of Bax, Bcl-2, NOTCH 1 and jagged 1 was estimated by real-time quantitative reverse transcription PCR (qRT-PCR). cell cycle phase distribution was evaluated Annexin V-FITC / PI staining.


Results: MTT assay showed a dose and time dependent cytoxicity of andrographolide on SW-480 cells. It also inhibits migratory and colony forming potential of SW-480 cells. In addition, andrografolida also showed impaired mitochondrial membrane potential and induced apoptosis through nuclear condensation. Flow cytometric evaluation showed improved early and late andrographolide cells induced apoptosis and upregulation of proapoptotic (Bax and Bad) and downregulation of Bcl-2 antiapoptotic in SW-480 treated cells. Andrographolide plus intracellular ROS and induced G0 / G1 phase cell cycle arrest in SW480 colon cancer cells. Furthermore, andrografolida suppress Notch signaling by reducing the expression of NOTCH 1 and jagged one.
Conclusions: Our findings indicate that the andrographolide constraints SW-480 cell growth through inhibition of the Notch signaling pathway.

Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway
Andrographolide Inhibits Proliferation of Colon Cancer SW-480 Cells via Downregulating Notch Signaling Pathway

MicroRNA-133b exacerbate atherosclerosis by activating the Notch signaling pathway1

The purpose of this study was to determine the effect of miR-133b in atherosclerosis (AS). A rat model of AS (AS Group) was established, and serum levels of total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein (LDL) cholesterol were detected. The thoracic aorta tissue was subjected to hematoxylin and eosin staining for pathological examination. Mice were intravenously injected with microRNA (mir) -133b mimic (group miR-133b mimic + US) and miR-133b mimic negative control (miR-133b group NC + US).

Normal mice given the name of Sham group. reconstruction parameters of vascular, inflammatory factors Ratio Collagen / Vascular Area (CA / CVA) and serum of mice in each group were detected. MRNA expression was measured by quantitative reverse transcription-PCR and protein expression was determined by western blot analysis. An in vitro model of the US induced in vascular smooth muscle cells (VSMC) using oxidized (ox) -LDL. CCK-8 and wound healing tests used to detect cell proliferation and migration.

Compared with Sham group, the rats of the US group, AS + miR-133b mimic NC group and the group AS + miR-133b have a higher intima thickness (IT), tumor necrosis factor (TNF) -α and monocytes kemoatraktan protein (MCP) -1 level, as well as increased expression of Notch1 and Jagged1; and they have a lower medial thickness (MT), the ratio CA / CVA and Notch3 expression (all P <0.05).

iFluor™ 610 succinimidyl ester

1038 1 mg
EUR 219

iFluor™ A7 SE

1039 1 mg
EUR 219

iFluor™ 546 succinimidyl ester

1048 1 mg
EUR 219

iFluor™ 568 succinimidyl ester

1049 1 mg
EUR 219

iFluor™ 450 maleimide

1057 1 mg
EUR 219

iFluor™ 647 amine

1074 1 mg
EUR 219

iFluor™ 680 amine

1076 1 mg
EUR 219

iFluor™ 700 amine

1077 1 mg
EUR 219

iFluor™ 710 amine

1078 1 mg
EUR 219

iFluor™ 750 amine

1079 1 mg
EUR 219

iFluor™ 680 hydrazide

1086 1 mg
EUR 219

iFluor™ 700 hydrazide

1087 1 mg
EUR 219

iFluor™ 750 hydrazide

1088 1 mg
EUR 219

ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11001 1 L
EUR 202

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11003 1 L
EUR 480

ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*

11004 5x10 mL
EUR 132

Amplite™ Blue

11005 25 mg
EUR 176

Amplite™ IR

11009 1 mg
EUR 132

Amplite™ Red

11011 1000 Assays
EUR 132

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11012 100 ml
EUR 115

ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1105 2 Labelings
EUR 176

Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*

11050 1 g
EUR 115

iFluor™ 488 tyramide

11060 1 mg
EUR 219

Azido-Cy5 tyramide

11061 1 mg
EUR 306

Amplite™ Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*

11100 200 Tests
EUR 219

Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

11105 500 tests
EUR 219

ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1114 2 Labelings
EUR 176

ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1120 2 Labelings
EUR 176

ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1123 2 Labelings
EUR 176

ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1126 2 Labelings
EUR 50

Amplite™ Colorimetric Glucose Oxidase Assay Kit

11299 500 Tests
EUR 306

ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1130 2 Labelings
EUR 176

Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

11300 500 Tests
EUR 219

Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

11301 200 Tests
EUR 219

Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

11302 200 Tests
EUR 219

Amplite™ Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

11303 200 Tests
EUR 219

Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

11304 200 Tests
EUR 219

Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit

11305 200 Tests
EUR 219

Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence*

11306 200 Tests
EUR 219

Amplite™ Colorimetric Xanthine Oxidase Assay Kit

11307 200 Tests
EUR 219

mFluor™ Violet 450 acid

1140 5 mg
EUR 219

Amplite™ Colorimetric Acetylcholinesterase Assay Kit

11400 200 Tests
EUR 219

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

11401 200 Tests
EUR 219

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*

11402 200 Tests
EUR 219

Amplite™ Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*

11403 200 Tests
EUR 219

mFluor™ Violet 510 acid

1141 5 mg
EUR 306

mFluor™ Violet 540 acid

1142 5 mg
EUR 306

mFluor™ Blue 570 acid

1143 5 mg
EUR 306

mFluor™ Green 620 acid

1144 5 mg
EUR 306

mFluor™ Yellow 630 acid

1145 5 mg
EUR 50

mFluor™ Red 700 acid

1146 5 mg
EUR 306

mFluor™ Violet 500 SE

1149 1 mg
EUR 219

mFluor™ Violet 450 SE

1150 1 mg
EUR 219

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*

11503 200 Tests
EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*

11504 100 Tests
EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*

11505 100 Tests
EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

11506 100 Tests
EUR 263

mFluor™ Violet 510 SE

1151 1 mg
EUR 219

mFluor™ Violet 540 SE

1152 1 mg
EUR 219

Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

11540 1000 Tests
EUR 219

Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

11541 1000 Tests
EUR 219

Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

11551 500 Tests
EUR 219

Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

11552 500 Tests
EUR 219

Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*

11553 500 Tests
EUR 306

Amplite™ Luminometric Peroxidase (HRP) Assay Kit

11559 500 Tests
EUR 219

Amplite™ Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

11560 200 Tests
EUR 306

mFluor™ Blue 570 SE

1160 1 mg
EUR 306

FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*

11600 5 mg
EUR 132

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

11610 25 mg
EUR 115

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

11612 10 g
EUR 898

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

11614 25 mg
EUR 115

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

11617 5 g
EUR 480

pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9*

11619 25 mg
EUR 115

CF-MUP, sodium salt *Superior alternative to MUP*

11628 10 mg
EUR 50

mFluor™ Green 620 SE

1165 1 mg
EUR 306

mFluor™ Yellow 630 SE

1170 1 mg
EUR 50

ADP-ribose-pNP

11700 1 mg
EUR 219

mFluor™ Red 780 SE

1191 1 mg
EUR 306

Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

11950 500 Tests
EUR 176

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

11952 500 Tests
EUR 219

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

11953 500 Tests
EUR 219

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence*

11954 500 Tests
EUR 219

Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence*

11956 100 Tests
EUR 219

ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1227 2 Labelings
EUR 176

ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1235 2 Labelings
EUR 176

ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1240 2 Labelings
EUR 176

ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1245 2 Labelings
EUR 176

Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]

12481 25 mg
EUR 219

Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]

12482 100 mg
EUR 480

ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1250 2 Labelings
EUR 176

D-Luciferin, free acid *CAS#: 2591-17-5*

12501 25 mg
EUR 115

D-Luciferin, free acid *CAS#: 2591-17-5*

12502 100 mg
EUR 132

D-Luciferin, free acid *CAS#: 2591-17-5*

12503 1 g
EUR 528

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12505 25 mg
EUR 115

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12506 100 mg
EUR 132

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12507 1 g
EUR 263

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12509 25 mg
EUR 115

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12510 100 mg
EUR 132

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12511 1 g
EUR 528

D-Luciferin phosphate *CAS 145613-12-3*

12512 1 mg
EUR 219

D-Luciferin galactoside *CAS 131474-38-9*

12513 5 mg
EUR 219

D-Luciferin methyl ester *CAS 73918-26-0*

12514 5 mg
EUR 219

D-Luciferin ethyl ester *CAS 135251-85-3*

12515 5 mg
EUR 219

D-Luciferin acetate

12516 5 mg
EUR 219

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12518 1 plate
EUR 176

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12519 10 plates
EUR 393

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12520 100 plates
EUR 2616

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12530 1 plate
EUR 219

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12531 10 Plates
EUR 702

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12532 100 plates
EUR 3486

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12535 1 plate
EUR 219

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12536 10 plates
EUR 876

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12537 100 plates
EUR 3486

ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1255 2 Labelings
EUR 176

Amplite™ Colorimetric Beta-Lactamase Activity Assay Kit

12551 200 Tests
EUR 393

BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]

12560 1 mg
EUR 219

BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]

12561 5 mg
EUR 393

ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1260 2 Labelings
EUR 176

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

12601 500 Tests
EUR 306

Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

12602 200 Tests
EUR 306

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*

12603 200 Tests
EUR 306

Amplite™ Colorimetric Beta-Galactosidase Assay Kit

12604 200 Tests
EUR 306

HIS Lite™ Cy3 Bis NTA-Ni Complex

12610 1 mg
EUR 393

HIS Lite™ Cy5 Bis NTA-Ni Complex

12613 1 mg
EUR 393

ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1265 2 Labelings
EUR 176

ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1290 2 Labelings
EUR 176

ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1292 2 Labelings
EUR 176

ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1294 2 Labelings
EUR 176

ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1299 2 Labelings
EUR 176

ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*

1300 2 Labelings
EUR 876

Benzoyl-Leu-Gly-Arg-Aminoluciferin

13000 1 mg
EUR 480

BOC-Leu-Gly-Arg-Aminoluciferin

13001 1 mg
EUR 480

BOC-Val-Pro-Arg-Aminoluciferin

13002 1 mg
EUR 480

D-VLK-AMC [D-Val-Leu-Lys-AMC]

13201 5 mg
EUR 132

Ac-DEVD-AFC *CAS 201608-14-2*

13401 5 mg
EUR 132

Ac-DEVD-AMC *CAS 169332-61-0*

13402 5 mg
EUR 132

Ac-DEVD-CHO *CAS 169332-60-9*

13403 1 mg
EUR 132

Ac-DEVD-pNA *CAS 189950-66-1*

13405 5 mg
EUR 132

Ac-IETD-AFC *CAS 211990-57-7*

13410 5 mg
EUR 219

Ac-IETD-AMC

13411 5 mg
EUR 219

Ac-IETD-CHO *CAS 191338-86-0*

13412 5 mg
EUR 219

Z-IETD-pNA *CAS 219138-21-3*

13413 5 mg
EUR 219

D-Aminoluciferin

13415 10 mg
EUR 219

Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*

1342 2 Labelings
EUR 306

Z-DEVD-AMC

13421 5 mg
EUR 176

Z-IETD-AFC *CAS 219138-02-0*

13425 5 mg
EUR 158

Ac-LEHD-AMC *CAS 292633-16-0*

13426 5 mg
EUR 176

(Ac-LEHD)2-R110

13427 1 mg
EUR 132

(Ac-IETD)2-R110

13431 1 mg
EUR 132

Z-DEVD-ProRed™ 620

13433 1 mg
EUR 306

Z-IETD-ProRed™ 620

13434 1 mg
EUR 306

Z-LEHD-ProRed™ 620

13435 1 mg
EUR 306

Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8*

13445 10 mg
EUR 176

Gly-Pro-AMC *CAS 115035-46-6*

13450 5 mg
EUR 132

In addition, miR-133b mimic promoted proliferation and migration, Notch1 and Jagged1 upregulated and downregulated Notch3 in VSMC ox-LDL-induced. Taken together, miR-133b worsen the US by activating Notch signaling pathway, which could serve as a potential target for the treatment of AS.

Anthony

Related Posts

fallback-image

IMMUNOGLOBULINS

Increased Expression of Fibulin-1 Is Associated With Hepatocellular Carcinoma Progression by Regulating the Notch Signaling Pathway

Increased Expression of Fibulin-1 Is Associated With Hepatocellular Carcinoma Progression by Regulating the Notch Signaling Pathway

Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain

Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain

TWIST1 correlates with Notch signaling pathway to develop esophageal squamous cell carcinoma

TWIST1 correlates with Notch signaling pathway to develop esophageal squamous cell carcinoma

No Comment

Leave a Reply

Your email address will not be published.

Tags

October 2022
M T W T F S S
 12
3456789
10111213141516
17181920212223
24252627282930
31  

Recent Posts

Categories

Pages