Fibulin-1, a component of the extracellular matrix (ECM), which is a prognostic, pathophysiological and diagnostic role in hepatocellular carcinoma (HCC) is still unexplored. We first found that both Fibulin-1 RNA (mRNA) or protein levels were greatly elevated in HCC tissue compared to normal tissue. Fibulin-1 correlated with overall survival is poor, and it is an independent prognostic predictor (p = 0.001). Furthermore, the Expression or Fibulin-1 inhibition is reduced or sensitized HCC cell apoptosis signal, and Fibulin-1 gag pressed HCC cells’ ability to form tumors in vivo.
Additionally, Fibulin-1 apoptosis is inhibited through the Notch while Fibulin-1 silencing no effect evident in the p-MAPK, p-c-jun and the expression of p-STAT3, and both Mcl-1 and Bcl-xL is the target of Fibulin-1. Furthermore, stromal and immune scores increased in high Fibulin-1 network and FBLN1 expression is associated with increased macrophage infiltration using xcell, TIMER and TISDIB based database tool TCGA HCC.
Importantly, RNA (cfRNA) cell-free circulating levels of Fibulin-1 in serum increased significantly in patients with HCC compared with those in healthy controls, individuals with chronic hepatitis B and patients with HBV-induced liver cirrhosis. The area under the receiver operating characteristic curve (AUC) was 0.791 for Fibulin-1, 0.640 for α-fetoprotein and 0.868 for the combination of the two tumor markers. Our findings indicate that Fibulin-1 may be a potential prognostic indicator, promising serum biomarkers and therapeutic targets in patients with HCC.
Increased Expression of Fibulin-1 Is Associated With Hepatocellular Carcinoma Progression by Regulating the Notch Signaling Pathway
Down-regulation of brain UBA6 aggravate the injury by inhibiting the activation of Notch signaling pathway to promote brain cell apoptosis in rat model of acute cerebral infarction
This study aims to examine the role UBA6 in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 dataset, which is downloaded from GEO database. Venn methods to identify common degs. 68 up-regulated and 51 degs overlap down-regulated degs overlaps used to build a network of PPI by STRING online database. UBA6 identified as a gene hub with CytoHubba of Cytoscape plugin. GO and KEGG pathway enrichment analysis was performed using the DAVID website online.
UBA6 knockout aggravated MCAO-mediated brain injury and cell apoptosis in rat brain tissue by H & E and TTC staining and TUNEL assay. The results of flow cytometry and western blot tests further indicated that inhibition of hippocampal neurons UBA6 induce apoptosis and increased cleaved-caspase-3/9 protein levels. Notch1 protein level, NICD and Hes1 suppressed by down-regulated UBA6. UBA6 is a low expression in the poor prognosis group of 100 patients with ACI. Logistic regression analysis showed that hypertension, blood sugar, a dose of urokinase,
Description: A sandwich ELISA kit for quantitative measurement of Human EDN (Eosinophil-Derived Neurotoxin) in samples from Serum, Plasma, Cell supernatant
Human Eosinophil-derived neurotoxin,EDN/RNASE2 ELISA kit
Description: A polyclonal antibody against Botulinum neurotoxin type C1. Recognizes Botulinum neurotoxin type C1 from Clostridium botulinum. This antibody is Unconjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Botulinum neurotoxin type E. Recognizes Botulinum neurotoxin type E from Clostridium butyricum. This antibody is Unconjugated. Tested in the following application: ELISA
Description: Quantitativesandwich ELISA kit for measuring Human ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Human ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A polyclonal antibody against Botulinum neurotoxin type C1. Recognizes Botulinum neurotoxin type C1 from Clostridium botulinum. This antibody is HRP conjugated. Tested in the following application: ELISA
Botulinum neurotoxin type E Antibody, HRP conjugated
Description: A polyclonal antibody against Botulinum neurotoxin type E. Recognizes Botulinum neurotoxin type E from Clostridium butyricum. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Botulinum neurotoxin type C1. Recognizes Botulinum neurotoxin type C1 from Clostridium botulinum. This antibody is FITC conjugated. Tested in the following application: ELISA
Botulinum neurotoxin type E Antibody, FITC conjugated
Description: A polyclonal antibody against Botulinum neurotoxin type E. Recognizes Botulinum neurotoxin type E from Clostridium butyricum. This antibody is FITC conjugated. Tested in the following application: ELISA
Clostridium botulinum Botulinum neurotoxin type B (botB)
Description: A polyclonal antibody against Botulinum neurotoxin type C1. Recognizes Botulinum neurotoxin type C1 from Clostridium botulinum. This antibody is Biotin conjugated. Tested in the following application: ELISA
Botulinum neurotoxin type E Antibody, Biotin conjugated
Description: A polyclonal antibody against Botulinum neurotoxin type E. Recognizes Botulinum neurotoxin type E from Clostridium butyricum. This antibody is Biotin conjugated. Tested in the following application: ELISA
Micrurus tener tener Kunitz-type neurotoxin MitTx-alpha
Description: Quantitativesandwich ELISA kit for measuring Mouse ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Multiple organ tumor with neuroendocrine differentiation tissue array, including pathology grade, TNM and clinical stage, 36 cases/72 cores
Multiple organs neuroendocrine tumor tissue array with normal tissue
Description: Multi-normal human tissues array, representing FDA guideline for antibody cross-reactivity testing, 32 types of human organs, 99 cores, each type taken from 3 different individuals (core size 1.5mm).
UBA6 expression and AF is a major risk factor for poor outcome after thrombolytic therapy for patients with ACI. ROC curve analysis showed that the sensitivity and specificity UBA6 well (sensitivity 100%, specificity 89%, and AUC = 0.772) which will be used to evaluate the poor prognosis of ACI. In conclusion, intensive UBA6 down-regulated MCAO-induced brain injury by inhibiting the activation of Notch signaling pathway to promote apoptosis of neurons of the hippocampus and used to predict poor prognosis of ACI.
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Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Equipments | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | quipments | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Uncategorized | Vector & Virus | Western Blot 
Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Equipments | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | quipments | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Uncategorized | Vector & Virus | Western Blot
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