elegans lin-12, informed by the first oncogenic mutation affecting a human Notch gene
SPON2 Is Upregulated through Notch Signaling Pathway and Promotes Tumor Progression in Gastric Cancer
Spondin-2 (SPON2) involved in cancer development and metastasis of many tumors; However, the role and the underlying mechanisms in gastric cancer is still unclear. In this study, we examined the role SPON2 and associated signaling pathway in gastric cancer progression and metastasis. SPON2 expression level was found to be regulated in gastric cancer cell lines and tissues of patients compared to gastric epithelial cells of normal and normal controls.
Furthermore, SPON2 silencing was observed to reduce cell proliferation and motility and reduce tumor growth in xenograft mice. Conversely, excess SPON2 found to increase cell proliferation and motility. Next, we focus on regulatory mechanisms SPON2 in gastric cancer. cDNA microarray and in vitro studies suggest that Notch signaling was significantly correlated with the expression SPON2.
Therefore, we confirm how Notch signaling pathway regulates the expression of Notch-related SPON2 using signaling interactions of transcription factors and reporter gene assay. In addition, activation of Notch signaling is observed to increase cell proliferation, migration, and invasion through SPON2 expression. Our study suggests that Notch signaling-mediated upregulation SPON2 associated with aggressive development of gastric cancer. In conclusion, we recommend SPON2 regulated by Notch signaling as a potential target gene to inhibit the development of gastric cancer.
SPON2 Is Upregulated through Notch Signaling Pathway and Promotes Tumor Progression in Gastric Cancer
PNO1, which negatively regulated by miR-340-5p, promoting the development of adenocarcinoma of the lung through the Notch signaling pathway
Many studies have shown that hyperactivation of ribosome biogenesis plays an important role in the initiation and progression of cancer. As the ribosome assembly factor, PNO1 play an important role in ribosome biogenesis. However, little is known about the expression and function in human tumors PNO1. In our study, we aimed to explore the functional role and molecular mechanisms that underlie PNO1 in human lung adenocarcinoma (LUAD). Both bioinformatics databases and tumor tissues showed that expression in tissues LUAD PNO1 higher than in the adjacent tissue and predicted poor survival in patients LUAD. In vitro and in vivo tests showed that downregulation of the expression of PNO1 suppressed cell proliferation and invasion LUAD. Further research found that miR-340-5p PNO1 depressed expression via direct binding to the 3 ‘translated region (UTR) of PNO1.
PNO1 expression negatively correlated with miR-340-5p LUAD expression in cells and tissue samples. Moreover, upregulation or downregulation of the expression of miR-340-5p reversed the inhibitory effects and excessive PNO1, respectively. Meanwhile, downregulation of Notch signaling pathway inhibits PNO1 modulated epithelial mesenchymal transition (EMT). These results indicate that PNO1, negatively regulated by miR-340-5p, plays an important role in the development of LUAD through the Notch signaling pathway. The miR-340-5p / PNO1 / Notch axis may be a potential target for the treatment of the individual and the right of patients LUAD in the future. Forty Kunming mice were randomly divided into normal group (n = 10) and model group (n = 30).
Description: Multi-normal human tissues array, representing FDA guideline for antibody cross-reactivity testing, 32 types of human organs, 99 cores, each type taken from 3 different individuals (core size 1.5mm).
The Multiplex Human Micronutrient Array (7-plex) is a fully quantitative chemiluminescent assay allowing concurrent measurement of biomarkers used in nutritional assessment in serum or heparin plasma samples. Analytes CRP, Ferritin, HRP2, sTfR, and Thyroglobulin (Tg) are sandwich ELISAs while AGP and RBP4 are competitive ELISAs. This product is alternatively known in previous publications as Multiplex Micronutrient Assessment Tool (MMAT).
A Human Micronutrient Assay kit contains a 96-well plate, with each well featuring the 7-plex along with reagents necessary to perform the test. Our high quality reagents help ensure the accuracy of your results.
Using just 5 µl of sample per well, up to 80 wells can be tested for all seven markers in the panel in 3.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost effective means of generating a biomarker profile for each sample.
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Assay Ranges
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Analytes
Assay Types
Calibrator Range
Upper Limit of Quantification (ULOQ)
Lower Limit of Quantification (LLOQ)
Limit of Detection
AGP
Competitive
0.36-0.0005 (g/L)
0.25 (g/L)
0.0019 (g/L)
0.00096 (g/L)
CRP
Sandwich
1-0.0014 (mg/L)
0.7 (mg/L)
0.0018 (mg/L)
0.00089 (mg/L)
Ferritin
Sandwich
23-0.032 (µg/L)
16.17 (µg/L)
0.041 (µg/L)
0.021 (µg/L)
HRP2
Sandwich
1.9-0.0026 (µg/L)
1.33 (µg/L)
0.0034 (µg/L)
0.0017 (µg/L)
RBP4
Competitive
1.01-0.0014 (µmol/L)
0.71 (µmol/L)
0.0054 (µmol/L)
0.0027 (µmol/L)
sTFR
Sandwich
4-0.0055 (mg/L)
2.8 (mg/L)
0.0071 (mg/L)
0.0036 (mg/L)
Thyroglobulin
Sandwich
15.1-0.021 (µg/L)
10.57 (µg/L)
0.027 (µg/L)
0.013 (µg/L)
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Kit Components
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Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
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Reagents Include:
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Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Calibrator Additive: Purified ferritin antigen in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;Competitor: Lyophilized, biotinylated competitive antigen;Substrate A: Liquid, 3.5 mL/vial;Substrate B: Liquid, 3.5 mL/vial;Sample Diluent (2X): Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Colon cancer tissue array, with adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 50 cases/90 cores, replacing CO901
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Model of ulcerative colitis induced by free drinking of 2% dextran sulfate sodium (DSS). After 15 days, rats were divided into model group, 6-gingerenol group and positive control group with 10 rats in each group. normal group and model group were treated with normal saline, 6-gingerenol group treated with 6-shogaol 100 mg / (kg · d), the positive control group treated with sulfasalazine 100 mg / (kg · d) for 20 days. Intestinal histopathological changes were observed, and the expression of Hes-1 and Mathematics-1protein in colonic epithelial cells was detected by double immunofluorescence labeling method.
