elegans lin-12, informed by the first oncogenic mutation affecting a human Notch gene
SPON2 Is Upregulated through Notch Signaling Pathway and Promotes Tumor Progression in Gastric Cancer
Spondin-2 (SPON2) involved in cancer development and metastasis of many tumors; However, the role and the underlying mechanisms in gastric cancer is still unclear. In this study, we examined the role SPON2 and associated signaling pathway in gastric cancer progression and metastasis. SPON2 expression level was found to be regulated in gastric cancer cell lines and tissues of patients compared to gastric epithelial cells of normal and normal controls.
Furthermore, SPON2 silencing was observed to reduce cell proliferation and motility and reduce tumor growth in xenograft mice. Conversely, excess SPON2 found to increase cell proliferation and motility. Next, we focus on regulatory mechanisms SPON2 in gastric cancer. cDNA microarray and in vitro studies suggest that Notch signaling was significantly correlated with the expression SPON2.
Therefore, we confirm how Notch signaling pathway regulates the expression of Notch-related SPON2 using signaling interactions of transcription factors and reporter gene assay. In addition, activation of Notch signaling is observed to increase cell proliferation, migration, and invasion through SPON2 expression. Our study suggests that Notch signaling-mediated upregulation SPON2 associated with aggressive development of gastric cancer. In conclusion, we recommend SPON2 regulated by Notch signaling as a potential target gene to inhibit the development of gastric cancer.
SPON2 Is Upregulated through Notch Signaling Pathway and Promotes Tumor Progression in Gastric Cancer
PNO1, which negatively regulated by miR-340-5p, promoting the development of adenocarcinoma of the lung through the Notch signaling pathway
Many studies have shown that hyperactivation of ribosome biogenesis plays an important role in the initiation and progression of cancer. As the ribosome assembly factor, PNO1 play an important role in ribosome biogenesis. However, little is known about the expression and function in human tumors PNO1. In our study, we aimed to explore the functional role and molecular mechanisms that underlie PNO1 in human lung adenocarcinoma (LUAD). Both bioinformatics databases and tumor tissues showed that expression in tissues LUAD PNO1 higher than in the adjacent tissue and predicted poor survival in patients LUAD. In vitro and in vivo tests showed that downregulation of the expression of PNO1 suppressed cell proliferation and invasion LUAD. Further research found that miR-340-5p PNO1 depressed expression via direct binding to the 3 ‘translated region (UTR) of PNO1.
PNO1 expression negatively correlated with miR-340-5p LUAD expression in cells and tissue samples. Moreover, upregulation or downregulation of the expression of miR-340-5p reversed the inhibitory effects and excessive PNO1, respectively. Meanwhile, downregulation of Notch signaling pathway inhibits PNO1 modulated epithelial mesenchymal transition (EMT). These results indicate that PNO1, negatively regulated by miR-340-5p, plays an important role in the development of LUAD through the Notch signaling pathway. The miR-340-5p / PNO1 / Notch axis may be a potential target for the treatment of the individual and the right of patients LUAD in the future. Forty Kunming mice were randomly divided into normal group (n = 10) and model group (n = 30).
Description: Multi-normal human tissues array, representing FDA guideline for antibody cross-reactivity testing, 32 types of human organs, 99 cores, each type taken from 3 different individuals (core size 1.5mm).
Description: Quantitativesandwich ELISA kit for measuring Human Nephronectin (NPNT) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Nephronectin(NPNT) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Nephronectin (NPNT) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Nephronectin (NPNT) in samples from tissue homogenates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nephronectin (NPNT) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nephronectin (NPNT) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nephronectin (NPNT) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nephronectin (NPNT) in Tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Nephronectin (NPNT) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: A sandwich ELISA kit for detection of Nephronectin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Nephronectin (NPNT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Nephronectin (NPNT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Nephronectin (NPNT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Amino Particle Array KitGentaur offers a wide variety of uniform particles, ranging in size from 0.05 um to 20 um, with surface functional groups such as carboxyl or amino groups for covalent coupling of proteins or other ligands.
Description: Colon cancer tissue array, with adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 50 cases/90 cores, replacing CO901
Description: Vulva cancer tissue array, including TNM, clinical stage and pathology diagnosis, replacing UV241
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Model of ulcerative colitis induced by free drinking of 2% dextran sulfate sodium (DSS). After 15 days, rats were divided into model group, 6-gingerenol group and positive control group with 10 rats in each group. normal group and model group were treated with normal saline, 6-gingerenol group treated with 6-shogaol 100 mg / (kg · d), the positive control group treated with sulfasalazine 100 mg / (kg · d) for 20 days. Intestinal histopathological changes were observed, and the expression of Hes-1 and Mathematics-1protein in colonic epithelial cells was detected by double immunofluorescence labeling method.
