Notch signaling pathway mediates different biological processes including stem cell self-renewal, progenitor cell fate decisions, and terminal differentiation. TWIST1 play key roles in tumor development and metastasis through induces epithelial-mesenchymal transition (EMT). Expression of the transcription complex core Notch pathway and target genes, as well as excessive TWIST1, are closely associated with aggressive clinicopathological variables esophageal squamous cell carcinoma (ESCC). Here we aim to explain the possible functional crosstalk between TWIST1 and Notch pathways in ESCCs.
Correlation between TWIST1 and Notch target genes was analyzed in 50 ESCCs and corresponding normal tissue. Using retroviral system, forced expression TWIST1 established ESCC KYSE-30 cell line and the expression of genes Notch signaling was assessed. a significant correlation between the expression TWIST1 and HEY1 / by hey2 found in different pathological variables of ESCC poor prognosis.
TWIST1 induced expression in KYSE-30 cells led to an increase noteworthy of Notch pathway gene expression revealed TWIST1 gene regulatory role of Notch signaling in cells. Based on the existing correlation between the expression of genes TWIST1 and Notch pathway in different pathological features of patients with ESCC, and KYSE-30 cell line, we can estimate that TWIST1 involved in the aggressiveness of the disease through regulation of Notch signaling genes. To the best of knowledge, this is the first report describing the effect TWIST1 Notch cascade genes in ESCC
TWIST1 correlates with Notch signaling pathway to develop esophageal squamous cell carcinoma
3-O- (E) p-Coumaroyl betulinic acid has anti-cancer activity and inhibits the Notch signaling pathway in breast cancer cells and mammosphere
Activation of Notch signaling is associated with tumor aggressiveness, poor clinical outcomes and drug resistance in breast cancer patients. Targeting Notch signaling with small molecule inhibitors may be a better strategy for anticancer drug development. We identified 3-O- (E) p-Coumaroylbetulinic acid (CB) as a lead compound and a potent inhibitor of Notch signaling pathway. Treatment of human breast cancer MBA-MD-231 and T47D cells with CB resulted in dose-and time-dependent inhibition of cell viability and G0 / G1 phase cell cycle arrest.
This effect was associated with a sharp decrease in the expression of cyclin D1 and its activating partner, cyclin-dependent kinase 2 with a concomitant increase in the cyclin kinase inhibitor p21, operating in G1 phase of the cell cycle. CB treatment induced early apoptosis of breast cancer cells as evidenced by an increase in cleaved caspase-3, a decrease in Bcl-2 and survivin, a surge of reactive oxygen species and mitochondrial membrane potential interference. CB treatment Notch target gene is altered.
Hes1, Hey1 and E-cadherin at the mRNA and protein levels in a time-dependent manner along with a decrease in Notch promoter activity at IC50 concentrations. Furthermore, treatment CB decline mammosphere formation in MCF-7 cells by down-modulation of the Notch signaling pathway and suppression of self-renewal markers such as c-Myc, SOX-2 and CD44. Our findings indicate that the CB has anticancer activity in breast cancer cells and suppress the ability of self-renewal in mammosphere as a result of modulation of cell-cycle engine, impaired mitochondrial function, induction of apoptosis, and inhibition.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Amino Particle Array KitGentaur offers a wide variety of uniform particles, ranging in size from 0.05 um to 20 um, with surface functional groups such as carboxyl or amino groups for covalent coupling of proteins or other ligands.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: A sandwich ELISA for quantitative measurement of Rat Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Notch Homolog 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Notch-1 ELISA Kit| Mouse Neurogenic locus notch homolog protein
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens)
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Armenian Hamster monoclonal Notch4 antibody (PE)
Inhibiting Notch Notch signaling may be an effective way to prevent retinal fibrosis , This study is of clinical significance in developing treatments to prevent fibrosis in proliferative vitreoretinopathy, proliferative diabetic retinopathy and wet age-related macular degeneration.
 20 profiles)
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles 
Human Notch Signaling Pathway  20 profiles)
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles 
Mouse Notch Signaling Primer Library 
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 
Array Designer 
Nylon Array )
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) 
Green qPCR SuperMix 
Probe qPCR SuperMix 
Universal qPCR Mixture 
Notch 1 antibody 
Notch 2 antibody 
Notch 1 Antibody )
pGreenFire1-Notch (plasmid) )
pGreenFire1-Notch (virus) 
anti-Notch 2 
Green qPCR SuperMix UDG 
Top Green qPCR SuperMix 
Tip Green qPCR SuperMix 
Library Quantification qPCR SuperMix 
qPCR Control Template Kit 
Eva QPCR SuperMix Kit 
Pro QPCR SuperMix Kit 
QPCR Positive Control Kit 
Neural Lineage qPCR Profiler 
Regulatory RNA qPCR Profiler 
Lentivirus qPCR Quantification Kit 
Retrovirus qPCR Quantification Kit 
Amino Particle Array Kit 
Fluorescent Particle Array Kit 
Blue Particle Array Kit 
FDA Standard Tissue Array 
CA125 antibody array kit 
Ferritin antibody array kit 
Microalbuminuria antibody array kit 
Rat Notch Homolog 1 ELISA kit  Protein)
Rat Notch Homolog 4 (NOTCH4) Protein 
FDA Standard Frozen Tissue Array - Rat Normal 
Notch-1 ELISA Kit| Mouse Neurogenic locus notch homolog protein  Polyclonal Antibody (Rat))
Notch Homolog 4 (NOTCH4) Polyclonal Antibody (Rat) )
Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens) 
cDNA Synthesis SuperMix for qPCR )
HotTaq Probe qPCR Mix (ROX) )
HotTaq Probe qPCR Mix (Capillary) 
HotTaq Probe qPCR Universal Mix )
HotTaq EvaGreen qPCR Mix (ROX) )
HotTaq EvaGreen qPCR Mix (Capillary) 
HotTaq EvaGreen qPCR Universal Mix 
SYBR Green qPCR Master Mix 
RT Master Mix for qPCR 
Power Eva QPCR SuperMix Kit 
Methylamp MS-qPCR Fast Kit 
BrightGreen 5X qPCR MasterMix-iCycler 
TaqProbe 5X qPCR MasterMix-ROX 
TaqProbe 5X qPCR MasterMix-iCycler 
TaqProbe 5X qPCR MasterMix-Multiplex 
BrightGreen 5X qPCR MasterMix-ROX 
BrightGreen 2X qPCR MasterMix-iCycler 
KiloGreen 2X qPCR MasterMix-iCycler 
KiloGreen 2X qPCR MasterMix-ROX 
BrightGreen miRNA qPCR MasterMix-iCycler 
BrightGreen miRNA qPCR MasterMix-ROX 
TaqProbe 2X qPCR MasterMix-ROX 
TaqProbe 2X qPCR MasterMix-iCycler 
TaqProbe 2X qPCR MasterMix-Multiplex 
BrightGreen 2X qPCR MasterMix-ROX 
AceQ qPCR Probe Master Mix 
Notch 1 Blocking Peptide )
Notch 3 antibody (PE) )
Notch 4 antibody (PE) 
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Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Equipments | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | quipments | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Uncategorized | Vector & Virus | Western Blot
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