Notch signaling pathway mediates different biological processes including stem cell self-renewal, progenitor cell fate decisions, and terminal differentiation. TWIST1 play key roles in tumor development and metastasis through induces epithelial-mesenchymal transition (EMT). Expression of the transcription complex core Notch pathway and target genes, as well as excessive TWIST1, are closely associated with aggressive clinicopathological variables esophageal squamous cell carcinoma (ESCC). Here we aim to explain the possible functional crosstalk between TWIST1 and Notch pathways in ESCCs.
Correlation between TWIST1 and Notch target genes was analyzed in 50 ESCCs and corresponding normal tissue. Using retroviral system, forced expression TWIST1 established ESCC KYSE-30 cell line and the expression of genes Notch signaling was assessed. a significant correlation between the expression TWIST1 and HEY1 / by hey2 found in different pathological variables of ESCC poor prognosis.
TWIST1 induced expression in KYSE-30 cells led to an increase noteworthy of Notch pathway gene expression revealed TWIST1 gene regulatory role of Notch signaling in cells. Based on the existing correlation between the expression of genes TWIST1 and Notch pathway in different pathological features of patients with ESCC, and KYSE-30 cell line, we can estimate that TWIST1 involved in the aggressiveness of the disease through regulation of Notch signaling genes. To the best of knowledge, this is the first report describing the effect TWIST1 Notch cascade genes in ESCC
TWIST1 correlates with Notch signaling pathway to develop esophageal squamous cell carcinoma
3-O- (E) p-Coumaroyl betulinic acid has anti-cancer activity and inhibits the Notch signaling pathway in breast cancer cells and mammosphere
Activation of Notch signaling is associated with tumor aggressiveness, poor clinical outcomes and drug resistance in breast cancer patients. Targeting Notch signaling with small molecule inhibitors may be a better strategy for anticancer drug development. We identified 3-O- (E) p-Coumaroylbetulinic acid (CB) as a lead compound and a potent inhibitor of Notch signaling pathway. Treatment of human breast cancer MBA-MD-231 and T47D cells with CB resulted in dose-and time-dependent inhibition of cell viability and G0 / G1 phase cell cycle arrest.
This effect was associated with a sharp decrease in the expression of cyclin D1 and its activating partner, cyclin-dependent kinase 2 with a concomitant increase in the cyclin kinase inhibitor p21, operating in G1 phase of the cell cycle. CB treatment induced early apoptosis of breast cancer cells as evidenced by an increase in cleaved caspase-3, a decrease in Bcl-2 and survivin, a surge of reactive oxygen species and mitochondrial membrane potential interference. CB treatment Notch target gene is altered.
Hes1, Hey1 and E-cadherin at the mRNA and protein levels in a time-dependent manner along with a decrease in Notch promoter activity at IC50 concentrations. Furthermore, treatment CB decline mammosphere formation in MCF-7 cells by down-modulation of the Notch signaling pathway and suppression of self-renewal markers such as c-Myc, SOX-2 and CD44. Our findings indicate that the CB has anticancer activity in breast cancer cells and suppress the ability of self-renewal in mammosphere as a result of modulation of cell-cycle engine, impaired mitochondrial function, induction of apoptosis, and inhibition.
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth,
Description: A competitive ELISA for quantitative measurement of Rat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Agouti signaling protein(ASIP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Suppressors Of Cytokine Signaling 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Suppressors Of Cytokine Signaling 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Regulator Of G Protein Signaling 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) Protein
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Suppressor of cytokine signaling 1 in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Rat Suppressor of cytokine signaling 3
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Suppressor of cytokine signaling 3 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: The endoplasmic reticulum (ER) is an organelle composed of an interconnected network of tubules, vesicles and cisternae found in all eukaryotic cells. It is involved in several specialized processes such as protein translation, folding, and transport of proteins to be used in the cell membrane or secreted from the cell. Accumulation of malfolded proteins in the ER activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase, to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA, converting it to a potent UPR transcriptional activator. ER stress also causes the cleavage of ATF6, yielding a cytosolic fragment which migrates to the nucleus and together with XBP-1, activates transcription of UPR-responsive genes. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.;;For images please see PDF data sheet
Description: The substance Cyclopamine is a hedgehog signaling blocker. It is synthetically produced and has a purity of >98%. The pure substance is white solid which is soluble to 5 mM in ethanol.
Description: The substance Cyclopamine is a hedgehog signaling blocker. It is synthetically produced and has a purity of >98%. The pure substance is white solid which is soluble to 5 mM in ethanol.
Description: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GΒL, which stimulates TOR’s kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.;;For images please see PDF data sheet
Description: A sandwich quantitative ELISA assay kit for detection of Rat Suppressors Of Cytokine Signaling 1 (SOCS1) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Suppressors Of Cytokine Signaling 1 (SOCS1) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Suppressors Of Cytokine Signaling 2 (SOCS2) in samples from tissue homogenates or other biological fluids.
Rat Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Suppressors Of Cytokine Signaling 2 (SOCS2) in samples from tissue homogenates or other biological fluids.
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Suppressors Of Cytokine Signaling 3 (SOCS3) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Suppressors Of Cytokine Signaling 3 (SOCS3) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Suppressors of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 3 (SOCS3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 3 (SOCS3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 3 (SOCS3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 3 (SOCS3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Suppressors Of Cytokine Signaling 3 (SOCS3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Suppressors Of Cytokine Signaling 3(SOCS3)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 2 (SOCS2) in Tissue homogenates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 2 (SOCS2) in Tissue homogenates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 2 (SOCS2) in Tissue homogenates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 2 (SOCS2) in Tissue homogenates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Suppressors Of Cytokine Signaling 2 (SOCS2) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Rat Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 1 (SOCS1) in Tissue homogenates, cell lysates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 1 (SOCS1) in Tissue homogenates, cell lysates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 1 (SOCS1) in Tissue homogenates, cell lysates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Suppressors Of Cytokine Signaling 1 (SOCS1) in Tissue homogenates, cell lysates and other biological fluids.
Rat Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Suppressors Of Cytokine Signaling 1 (SOCS1) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Rat Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Regulator Of G Protein Signaling 6 (RGS6) in samples from tissue homogenates or other biological fluids.
Rat Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Regulator Of G Protein Signaling 6 (RGS6) in samples from tissue homogenates or other biological fluids.
Rat Regulator of G- protein signaling 7, Rgs7 ELISA KIT
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 9 (RGS9) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 9 (RGS9) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 9 (RGS9) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 9 (RGS9) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 9 (RGS9) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 9 (RGS9) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 9 (RGS9) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 9 (RGS9) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Regulator Of G Protein Signaling 9 (RGS9) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 6 (RGS6) in Tissue homogenates and other biological fluids.
Rat Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 6 (RGS6) in Tissue homogenates and other biological fluids.
Rat Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 6 (RGS6) in Tissue homogenates and other biological fluids.
Rat Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 6 (RGS6) in Tissue homogenates and other biological fluids.
Rat Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Regulator Of G Protein Signaling 6 (RGS6) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Rat Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 7 (RGS7) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 7 (RGS7) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 7 (RGS7) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Regulator Of G Protein Signaling 7 (RGS7) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Regulator Of G Protein Signaling 7 (RGS7) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Regulator Of G Protein Signaling 6(RGS6)ELISA Kit
Description: A competitive ELISA for quantitative measurement of Rat WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat WNT1 Inducible Signaling Pathway Protein 1 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat WNT1 Inducible Signaling Pathway Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Inhibiting Notch Notch signaling may be an effective way to prevent retinal fibrosis , This study is of clinical significance in developing treatments to prevent fibrosis in proliferative vitreoretinopathy, proliferative diabetic retinopathy and wet age-related macular degeneration.
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Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Equipments | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | quipments | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Uncategorized | Vector & Virus | Western Blot 
Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Equipments | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | quipments | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Uncategorized | Vector & Virus | Western Blot
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